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Product Info Summary
| SKU: | A09682 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-HNF4G Antibody
SKU/Catalog Number
A09682
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HNF4G Antibody catalog # A09682. Tested in WB,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HNF4G Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09682)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human HNF4G(NP_004124.4).
Reactive Species
A09682 is reactive to HNF4G in Human, Mouse, Rat
Calculated molecular weight
45.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09682 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
ICC/IF: 1:50-1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using HNF4G antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 15s.
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Immunofluorescence analysis of U-2 OS cells using HNF4G Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.
Specific Publications For Anti-HNF4G Antibody (A09682)
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