Product Info Summary
SKU: | A09982-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-hnRNP D/AUF1/HNRNPD Antibody Picoband™
SKU/Catalog Number
A09982-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-hnRNP D/AUF1/HNRNPD Antibody Picoband™ catalog # A09982-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-hnRNP D/AUF1/HNRNPD Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09982-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human hnRNP D/AUF1/HNRNPD recombinant protein (Position: E88-N246).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09982-1 is reactive to HNRNPD in Human, Mouse, Rat
Applications
A09982-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
37-45 kDa
Calculated molecular weight
38.434kDa
Background of AUF1
Heterogeneous nuclear ribonucleoprotein D0 (HNRNPD) also known as AU-rich element RNA-binding protein 1 (AUF1) is a protein that in humans is encoded by the HNRNPD gene. It is mapped to 4q21.22. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of HNRNPD using anti-HNRNPD antibody (A09982-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human HL-60 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human A431 whole cell lysates,
Lane 6: human HepG2 whole cell lysates,
Lane 7: human Caco-2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNRNPD antigen affinity purified polyclonal antibody (Catalog # A09982-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for HNRNPD at approximately 37-45KD. The expected band sizes for HNRNPD are at 37, 40, 42 and 45KD.
Click image to see more details
Figure 10. IHC analysis of HNRNPD using anti-HNRNPD antibody (A09982-1). HNRNPD was detected in paraffin-embedded section of mouse intestine tissue tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HNRNPD Antibody (A09982-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. Western blot analysis of HNRNPD using anti-HNRNPD antibody (A09982-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse thymus tissue lysates,
Lane 5: mouse SP20 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNRNPD antigen affinity purified polyclonal antibody (Catalog # A09982-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for HNRNPD at approximately 37-45KD. The expected band sizes for HNRNPD are at 37, 40, 42 and 45KD.
Click image to see more details
Figure 3. IHC analysis of HNRNPD using anti-HNRNPD antibody (A09982-1).
HNRNPD was detected in paraffin-embedded section of B lymphocytoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HNRNPD Antibody (A09982-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of HNRNPD using anti-HNRNPD antibody (A09982-1).
HNRNPD was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HNRNPD Antibody (A09982-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of HNRNPD using anti-HNRNPD antibody (A09982-1).
HNRNPD was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HNRNPD Antibody (A09982-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of HNRNPD using anti-HNRNPD antibody (A09982-1).
HNRNPD was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HNRNPD Antibody (A09982-1) overnight at 4°C.Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 7. Flow Cytometry analysis of A431 cells using anti-HNRNPD antibody (A09982-1).
Overlay histogram showing A431 cells stained with A09982-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HNRNPD Antibody (A09982-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 8. IF analysis of HNRNPD using anti-HNRNPD antibody (A09982-1).
HNRNPD was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HNRNPD Antibody (A09982-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 9. IF analysis of HNRNPD using anti-HNRNPD antibody (A09982-1).
HNRNPD was detected in paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-HNRNPD Antibody (A09982-1) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For HNRNPD (Source: Uniprot.org, NCBI)
Gene Name
HNRNPD
Full Name
Heterogeneous nuclear ribonucleoprotein D0
Weight
38.434kDa
Alternative Names
AUF1A; heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein1, 37kDa); heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA-binding protein1, 37kD); heterogeneous nuclear ribonucleoprotein D0; hnRNPD0; type A HNRNPD AUF1, AUF1A, HNRPD, P37, hnRNPD0 heterogeneous nuclear ribonucleoprotein D heterogeneous nuclear ribonucleoprotein D0|ARE-binding protein AUFI, type A|AU-rich element RNA binding protein 1, 37kDa|hnRNP D0
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HNRNPD, check out the HNRNPD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HNRNPD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-hnRNP D/AUF1/HNRNPD Antibody Picoband™ (A09982-1)
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No publications found for A09982-1
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Customer Reviews
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1 Reviews For Anti-hnRNP D/AUF1/HNRNPD Antibody Picoband™
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Immunocytochemistry for Anti-HnRNP D/AUF1/HNRNPD Antibody
Excellent
Application | Immunocytochemistry |
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Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Human Colorectal tissue |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 37°C for 30 minutes |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 1ug/ml |
Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | at 37°C for 30 min |
Verified Customer
Verified customer
Submitted 2020-04-29
Customer Q&As
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1 Customer Q&As for Anti-hnRNP D/AUF1/HNRNPD Antibody Picoband™
Question
We are currently using anti-hnRNP D/AUF1/HNRNPD antibody A09982-1 for human tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-05-17
Answer
The anti-hnRNP D/AUF1/HNRNPD antibody (A09982-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-17