Product Info Summary
SKU: | A05506 |
---|---|
Size: | 50µg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-HO-2 Hmox2 Antibody
View all HO-2/HMOX2 Antibodies
SKU/Catalog Number
A05506
Size
50µg
Form
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Description
Boster Bio Anti-HO-2 Hmox2 Antibody catalog # A05506. Tested in IP, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HO-2 Hmox2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05506)
Host
Rabbit
Contents
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Native rat HO-2 protein, isolated from rat testes.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05506 is reactive to HMOX2 in Human, Mouse, Rat
Applications
A05506 is guaranteed for IP, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
36.033kDa
Background of HO-2/HMOX2
Anti-Glycogen Synthase 1 pS641 antibody is validated by IHC, Western Blot and ELISA. Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3 that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S641. Phosphorylation of GS at S641 has been associated with Antiphospholipid Antibody Syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1:500, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Hmox2 using anti-Hmox2 antibody (A05506).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hmox2 antigen affinity purified polyclonal antibody (Catalog # A05506) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Hmox2.
Click image to see more details
Figure 2. IHC analysis of Hmox2 using anti-Hmox2 antibody (A05506).
Hmox2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Hmox2 Antibody (A05506) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Hmox2 using anti-Hmox2 antibody (A05506).
Hmox2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Hmox2 Antibody (A05506) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For HMOX2 (Source: Uniprot.org, NCBI)
Gene Name
HMOX2
Full Name
Heme oxygenase 2
Weight
36.033kDa
Superfamily
heme oxygenase family
Alternative Names
heme oxygenase (decycling) 2; heme oxygenase 2; HMOX2; HO2; HO-2; HO-2EC 1.14.99.3 HMOX2 HO-2 heme oxygenase 2 heme oxygenase 2|heme oxygenase (decycling) 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HMOX2, check out the HMOX2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HMOX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HO-2 Hmox2 Antibody (A05506)
Hello CJ!
No publications found for A05506
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-HO-2 Hmox2 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-HO-2 Hmox2 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-HO-2 Hmox2 Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-HO-2 antibody A05506. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-09
Question
Will anti-HO-2 antibody A05506 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
According to the expression profile of placenta, HMOX2 is highly expressed in placenta. So, it is likely that anti-HO-2 antibody A05506 will work for WB with placenta.
Boster Scientific Support
Answered: 2019-07-31
Question
I have a question about product A05506, anti-HO-2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-08-22
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05506 anti-HO-2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-22