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Product Info Summary
| SKU: | A00153 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Homeobox protein NANOG NANOG Antibody
SKU/Catalog Number
A00153
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Homeobox protein NANOG NANOG Antibody (Catalog # A00153). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NANOG antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Homeobox protein NANOG NANOG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00153)
Host
Rabbit
Contents
NANOG Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
NANOG antibody was raised against a 19 amino acid synthetic peptide near the center of human NANOG. The immunogen is located within amino acids 130 - 180 of NANOG.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Two alternatively spliced transcript variants have been observed.
Reactive Species
A00153 is reactive to Nanog in Human, Mouse, Rat
Applications
A00153 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
34.24kDa
Background of Nanog
Expression of NANOG is required for the maintenance of pluripotency in epiblast and embryonic stem (ES) cells as well as for the ability to maintain ES self-renewal independently of LIF/Stat3. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NANOG antibody can be used for detection of NANOG by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NANOG in human spleen tissue lysate with NANOG antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of NANOG in human spleen tissue with NANOG antibody at 5 μg/mL.
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Immunofluorescence of NANOG in Human Spleen cells with NANOG antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Nanog (Source: Uniprot.org, NCBI)
Gene Name
Nanog
Full Name
Homeobox protein NANOG
Weight
34.24kDa
Superfamily
Nanog homeobox family
Alternative Names
FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Nanog Nanog|2410002E02Rik, EN, ENK, ecat, ecat4|Nanog homeobox|homeobox protein NANOG|ES cell-associated protein 4|early embryo specific expression NK family|early embryo specific expression NK-type homeobox protein|homeobox transcription factor Nanog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Nanog, check out the Nanog Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nanog: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Homeobox protein NANOG NANOG Antibody (A00153)
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No publications found for A00153
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