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Product Info Summary
| SKU: | A06154 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Hox-A7 Antibody
SKU/Catalog Number
A06154
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Hox-A7 Antibody catalog # A06154. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hox-A7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06154)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human HOXA7. AA range:91-140
Reactive Species
A06154 is reactive to HOXA7 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
25.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06154 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western blot analysis of lysates from COLO205 and Jurkat cells, using HOXA7 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western Blot analysis of various cells using Hox-A7 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
Specific Publications For Anti-Hox-A7 Antibody (A06154)
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Customer Q&As
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6 Customer Q&As for Anti-Hox-A7 Antibody
Question
Is this A06154 anti-Hox-A7 antibody reactive to the isotypes of HOXA7?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
The immunogen of A06154 anti-Hox-A7 antibody is Synthesized peptide derived from the Internal region of human Hox-A7. at AA rangle: 60-140. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-23
Question
Will A06154 anti-Hox-A7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
As indicated on the product datasheet, A06154 anti-Hox-A7 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-06
Question
Is there a BSA free version of anti-Hox-A7 antibody A06154 available?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Hox-A7 antibody A06154 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-25
Question
I was wanting to use your anti-Hox-A7 antibody for WB for rat vagina on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat vagina identification?
Verified Customer
Verified customer
Asked: 2017-09-20
Answer
As indicated on the product datasheet, A06154 anti-Hox-A7 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat vagina in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-20
Question
See below the WB image, lot number and protocol we used for vagina using anti-Hox-A7 antibody A06154. Please let me know if you require anything else.
N. Baker
Verified customer
Asked: 2017-04-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-04-13
Question
We are currently using anti-Hox-A7 antibody A06154 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
B. Rodriguez
Verified customer
Asked: 2016-03-30
Answer
The anti-Hox-A7 antibody (A06154) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-03-30
