Anti-Hox-A7 Antibody

HOXA7 antibody

Boster Bio Anti-Hox-A7 Antibody catalog # A06154. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A06154
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Hox-A7 Antibody

View all HOXA7 Antibodies

SKU/Catalog Number

A06154

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Hox-A7 Antibody catalog # A06154. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Hox-A7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06154)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human Hox-A7. at AA rangle: 60-140

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06154 is reactive to HOXA7 in Human, Mouse, Rat

Applications

A06154 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

25.355kDa

Background of HOXA7

APC2, also known as Anaphase promoting complex subunit 2, APC2, Cyclosome subunit 2, and ANAPC2, is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/cyclosome protein complex promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitors, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains at least eleven subunits. The composition of APC is highly conserved in organisms from yeast to humans. APC2 is a cullin family member that interacts through the cullin domain with ANAPC11 and UBCH10.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For HOXA7 (Source: Uniprot.org, NCBI)

Gene Name

HOXA7

Full Name

Homeobox protein Hox-A7

Weight

25.355kDa

Superfamily

Antp homeobox family

Alternative Names

ANTP; homeo box A7; homeobox A7; Homeobox protein Hox 1.1; Homeobox protein Hox-1A; homeobox protein Hox-A7; HOX1.1; HOX1AHOX1 HOXA7 ANTP, HOX1, HOX1.1, HOX1A homeobox A7 homeobox protein Hox-A7|homeo box A7|homeobox protein HOX-1A|homeobox protein Hox 1.1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HOXA7, check out the HOXA7 Infographic

HOXA7 infographic

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In this infographic, you will see the following information for HOXA7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Hox-A7 Antibody

Question

Is this A06154 anti-Hox-A7 antibody reactive to the isotypes of HOXA7?

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

The immunogen of A06154 anti-Hox-A7 antibody is Synthesized peptide derived from the Internal region of human Hox-A7. at AA rangle: 60-140. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-23

Question

Will A06154 anti-Hox-A7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-06

Answer

As indicated on the product datasheet, A06154 anti-Hox-A7 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-06

Question

Is there a BSA free version of anti-Hox-A7 antibody A06154 available?

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Hox-A7 antibody A06154 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-25

Question

I was wanting to use your anti-Hox-A7 antibody for WB for rat vagina on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat vagina identification?

Verified Customer

Verified customer

Asked: 2017-09-20

Answer

As indicated on the product datasheet, A06154 anti-Hox-A7 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat vagina in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-09-20

Question

See below the WB image, lot number and protocol we used for vagina using anti-Hox-A7 antibody A06154. Please let me know if you require anything else.

N. Baker

Verified customer

Asked: 2017-04-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-04-13

Question

We are currently using anti-Hox-A7 antibody A06154 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?

B. Rodriguez

Verified customer

Asked: 2016-03-30

Answer

The anti-Hox-A7 antibody (A06154) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-03-30

Order DetailsPrice
A06154

100uL

$399

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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