Rabbit IgG polyclonal antibody for Homeobox protein Hox-A3(HOXA3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
|Reactivity||Human, Mouse, Rat|
|Product Name||Anti-HOXA3 Antibody
See all HOXA3 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for HOXA3 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. HOXA3 information: Molecular Weight: 46369 MW; Subcellular Localization: Nucleus.|
|Cite This Product||Anti-HOXA3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1602)|
|Specificity||Anti-HOXA3 Antibody (PA1602) reacts with Human, Mouse, Rat HOXA3, in native form and recombinant. Superfamily members of HOXA3 are not reactive to PA1602.|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human HOXA3(427-443aa HHPSQGRIQEAPKLTHL), identical to the related rat and mouse sequences.|
|Reactivity||Human, Mouse, Rat|
Our Boster Quality Guarantee for Anti-HOXA3 Antibody covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-HOXA3 Antibody (PA1602).Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-HOXA3 antibody, PA1602, Western blotting
All lanes: Anti HOXA3 (PA1602) at 0.5ug/ml
WB: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 46KD
Observed bind size: 46KD
Anti-HOXA3 antibody, PA1602, IHC(P)
IHC(P): Human Pancreatic Cancer Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||Homeobox protein Hox-A3|
|Alternative Names||Homeobox protein Hox-A3;Homeobox protein Hox-1E;HOXA3;HOX1E;|
|Molecular Weight||46369 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.|
|Research Areas||Human, Mouse, Rat
*You can search these to find other products in these research areas.
|Background||HOXA3(HOMEOBOX A3) is a protein that in humans is encoded by the HOXA3 gene, which is also part of the A cluster on chromosome 7. HOXA3 encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Its cytogenetic location is 7q15.2. In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. HOXA3 protein is able to complement Hoxd3 deficiency when expressed in the context of the Hoxd3 allele.|
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Publishing with Anti-HOXA3 Antibody (PA1602)? Please let us know so we can cite the reference in this product datasheet. Email us at [email protected]
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.
Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugationA: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: What should I use for negative control?A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signalA: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?A: You can find the immunogen sequence under "
Q: What is the expected band size? Why is it different than the observed band size?A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?A: Check our protocols under the tech support tab.