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Product Info Summary
| SKU: | PA1605 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Homeobox protein Hox-A6 HOXA6 Antibody Picoband®
SKU/Catalog Number
PA1605
BA3654-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Homeobox protein Hox-A6 HOXA6 Antibody catalog # PA1605. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Homeobox protein Hox-A6 HOXA6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1605)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human HOXA6, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1605 is reactive to HOXA6 in Human, Mouse, Rat
Observed Molecular Weight
43 kDa
Calculated molecular weight
26.3 kDa
Background of HOXA6
HOXA6 (HOMEOBOX A6), also known as HOX1B, MOUSE or HOMOLOG OF,is a protein that in humans is encoded by the HOXA6 gene, which is also part of the A cluster on chromosome 7. HOXA6 encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Its cytogenetic location is 7q15.2. In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1605 is guaranteed for WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.1-0.5μg/ml | Human, Rat, Mouse |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
Click image to see more details
Anti-HOXA6 antibody, PA1605, Western blotting
Lane 1: SW620 cell Lysate
Lane 2: SW620 cell Lysate
Lane 3: HELA cell Lysate
Lane 4: HT1080 cell Lysate
Lane 5: HT1080 cell Lysate
Specific Publications For Anti-Homeobox protein Hox-A6 HOXA6 Antibody Picoband® (PA1605)
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Customer Q&As
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7 Customer Q&As for Anti-Homeobox protein Hox-A6 HOXA6 Antibody Picoband®
Question
I was wanting to use your anti-HOXA6 antibody for WB for mouse left uterine tube on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse left uterine tube identification?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
It shows on the product datasheet, PA1605 anti-HOXA6 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse left uterine tube in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-05
Question
Is this PA1605 anti-HOXA6 antibody reactive to the isotypes of HOXA6?
L. Johnson
Verified customer
Asked: 2019-12-31
Answer
The immunogen of PA1605 anti-HOXA6 antibody is A synthetic peptide corresponding to a sequence in the middle region of human HOXA6(95-114aa KQRGPGDYLHFSPEQQYKPD), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-31
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for left uterine tube using anti-HOXA6 antibody PA1605. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-18
Question
Will PA1605 anti-HOXA6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
As indicated on the product datasheet, PA1605 anti-HOXA6 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-06
Question
Can you help my question with product PA1605, anti-HOXA6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-05-31
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1605 anti-HOXA6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-31
Question
We are currently using anti-HOXA6 antibody PA1605 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
M. Zhao
Verified customer
Asked: 2018-04-30
Answer
The anti-HOXA6 antibody (PA1605) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-30
Question
Does anti-HOXA6 antibody PA1605 work for WB with left uterine tube?
D. Collins
Verified customer
Asked: 2016-11-11
Answer
According to the expression profile of left uterine tube, HOXA6 is highly expressed in left uterine tube. So, it is likely that anti-HOXA6 antibody PA1605 will work for WB with left uterine tube.
Boster Scientific Support
Answered: 2016-11-11
