Anti-HP1 gamma Rabbit Monoclonal Antibody

Product Name

Anti-HP1 gamma Rabbit Monoclonal Antibody

View all CBX3 Antibodies

SKU/Catalog Number

M01142-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-HP1 gamma Rabbit Monoclonal Antibody catalog # M01142-1. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HP1 gamma Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01142-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ACGB-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human HP1 gamma

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01142-1 is reactive to CBX3 in Human, Mouse, Rat

Applications

M01142-1 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee

Observed Molecular Weight

21 kDa

Calculated molecular weight

20.811kDa

Background of CBX3

Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
FC 1:100

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of HP1 Gamma using anti-HP1 Gamma antibody (M01142-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human U-937 whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HP1 Gamma antigen affinity purified monoclonal antibody (Catalog # M01142-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HP1 Gamma at approximately 21 kDa. The expected band size for HP1 Gamma is at 21 kDa.

Gene/Protein Information For CBX3 (Source: Uniprot.org, NCBI)

Alternative Names

chromobox homolog 3 (Drosophila HP1 gamma); chromobox homolog 3; chromobox protein homolog 3; Drosophila); HECH; Heterochromatin protein 1 homolog gamma; heterochromatin protein HP1 gamma; heterochromatin-like protein 1; HP1 gamma homolog; HP1 gamma; HP1-GAMMA; Modifier 2 protein CBX3 HECH, HP1-GAMMA, HP1Hs-gamma chromobox 3 chromobox protein homolog 3|HP1 gamma homolog|chromobox homolog 3 (HP1 gamma homolog, Drosophila)|heterochromatin protein 1 homolog gamma|heterochromatin protein HP1 gamma|heterochromatin-like protein 1|modifier 2 protein

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