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Product Info Summary
| SKU: | A12283-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-HRP-3 HDGFL3 Antibody
SKU/Catalog Number
A12283-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-HRP-3 HDGFL3 Antibody catalog # A12283-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HRP-3 HDGFL3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12283-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human HDGFRP3. AA range:131-180
Reactive Species
A12283-1 is reactive to HDGFL3 in Human, Mouse, Rat
Calculated molecular weight
22.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12283-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of NIH-3T3, PC12 cells using HRP-3 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-HRP-3 HDGFL3 Antibody (A12283-1)
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Customer Q&As
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6 Customer Q&As for Anti-HRP-3 HDGFL3 Antibody
Question
Is this A12283-1 anti-HRP-3 antibody reactive to the isotypes of HDGFL3?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
The immunogen of A12283-1 anti-HRP-3 antibody is Synthesized peptide derived from the Internal region of human HRP-3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-31
Question
Is a blocking peptide available for product anti-HRP-3 antibody (A12283-1)?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
We do provide the blocking peptide for product anti-HRP-3 antibody (A12283-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-11-06
Question
We are currently using anti-HRP-3 antibody A12283-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
J. Moore
Verified customer
Asked: 2017-06-14
Answer
The anti-HRP-3 antibody (A12283-1) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-14
Question
Would anti-HRP-3 antibody A12283-1 work for IHC with lymphocyte?
J. Wu
Verified customer
Asked: 2015-12-03
Answer
According to the expression profile of lymphocyte, HDGFL3 is highly expressed in lymphocyte. So, it is likely that anti-HRP-3 antibody A12283-1 will work for IHC with lymphocyte.
Boster Scientific Support
Answered: 2015-12-03
Question
Is there a BSA free version of anti-HRP-3 antibody A12283-1 available?
V. Miller
Verified customer
Asked: 2015-06-12
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HRP-3 antibody A12283-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-06-12
Question
I see that the anti-HRP-3 antibody A12283-1 works with IHC, what is the protocol used to produce the result images on the product page?
N. Gonzalez
Verified customer
Asked: 2013-07-24
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-07-24
