Anti-Hsc70/HSPA8 Antibody Picoband™

Boster Bio Anti-Hsc70/HSPA8 Antibody Picoband™ catalog # PB9641. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9641
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-Hsc70/HSPA8 Antibody Picoband™

See all HSPA8/HSC71/Hsc70 products

SKU/Catalog Number

PB9641

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Hsc70/HSPA8 Antibody Picoband™ catalog # PB9641. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Hsc70/HSPA8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9641)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human Hsc70 recombinant protein (Position: Q520-A614). Human Hsc70 shares 98.9% amino acid (aa) sequence identity with both mouse and rat Hsc70.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9641 is reactive to HSPA8 in Human, Mouse, Rat

Applications

PB9641 is guaranteed for Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For HSPA8 (Source: Uniprot.Org, NCBI)

Uniprot ID

P11142

Gene ID

3312

Gene Name

HSPA8

Full Name

Heat shock cognate 71 kDa protein

Weight

70.898kDa

Superfamily

heat shock protein 70 family

Alternative Names

Heat shock 70 kDa protein 8; heat shock 70kD protein 8; heat shock 70kDa protein 8; heat shock cognate 71 kDa protein; heat shock cognate protein 54; heat shock cognate protein, 71-kDa; HSC54; HSC70; HSC70heat shock 70kd protein 10; HSC71; HSP71; HSP73; HSP73constitutive heat shock protein 70; HSPA10; HSPA8; LAP1; lipopolysaccharide-associated protein 1; LPS-associated protein 1; MGC131511; MGC29929; NIP71; N-myristoyltransferase inhibitor protein 71 HSPA8 HEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71 heat shock protein family A (Hsp70) member 8 heat shock cognate 71 kDa protein|LPS-associated protein 1|N-myristoyltransferase inhibitor protein 71|constitutive heat shock protein 70|epididymis luminal protein 33|epididymis secretory sperm binding protein Li 72p|heat shock 70kDa protein 8|heat shock 70kd protein 10|heat shock cognate protein 54|lipopolysaccharide-associated protein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on HSPA8, check out the HSPA8 Infographic

HSPA8 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for HSPA8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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16 Customer Q&As for Anti-Hsc70/HSPA8 Antibody Picoband™

Question

Is there a BSA free version of anti-Hsc70/HSPA8 antibody PB9641 available?

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Hsc70/HSPA8 antibody PB9641 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-29

Question

I was wanting to use your anti-Hsc70/HSPA8 antibody for WB for rat colon carcinoma ovarian carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat colon carcinoma ovarian carcinoma identification?

S. Carter

Verified customer

Asked: 2020-04-22

Answer

As indicated on the product datasheet, PB9641 anti-Hsc70/HSPA8 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat colon carcinoma ovarian carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-22

Question

I have a question about product PB9641, anti-Hsc70/HSPA8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-03-13

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9641 anti-Hsc70/HSPA8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-13

Question

Here is the WB image, lot number and protocol we used for colon carcinoma ovarian carcinoma using anti-Hsc70/HSPA8 antibody PB9641. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-03-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-11

Question

I see that the anti-Hsc70/HSPA8 antibody PB9641 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-02-24

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-02-24

Question

We have observed staining in mouse brain. Are there any suggestions? Is anti-Hsc70/HSPA8 antibody supposed to stain brain positively?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

Based on literature brain does express HSPA8. Based on Uniprot.org, HSPA8 is expressed in frontal cortex, placenta skin, embryonic kidney, colon carcinoma ovarian carcinoma, brain, cajal-retzius cell fetal brain cortex, lymphoblast, melanoma, leukemic t-cell, cervix carcinoma, cervix carcinoma erythroleukemia, liver, colon carcinoma, among other tissues. Regarding which tissues have HSPA8 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1286667
Cervix carcinoma, Pubmed ID: 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Placenta, and Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-12-05

Question

Will anti-Hsc70/HSPA8 antibody PB9641 work for WB with colon carcinoma ovarian carcinoma?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

According to the expression profile of colon carcinoma ovarian carcinoma, HSPA8 is highly expressed in colon carcinoma ovarian carcinoma. So, it is likely that anti-Hsc70/HSPA8 antibody PB9641 will work for WB with colon carcinoma ovarian carcinoma.

Boster Scientific Support

Answered: 2019-06-03

Question

Will PB9641 anti-Hsc70/HSPA8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

As indicated on the product datasheet, PB9641 anti-Hsc70/HSPA8 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-24

Question

I would like using your anti-Hsc70/HSPA8 antibody for regulation of protein import studies. Has this antibody been tested with western blotting on mouse thymus tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-11-14

Answer

Thanks for your inquiry. This PB9641 anti-Hsc70/HSPA8 antibody is validated on rat thymus tissue, liver tissue, tissue lysate, testis tissue, mouse thymus tissue, hela whole cell lysate, 293t whole cell lysate. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-11-14

Question

We are currently using anti-Hsc70/HSPA8 antibody PB9641 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2018-05-18

Answer

The anti-Hsc70/HSPA8 antibody (PB9641) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-05-18

Question

We need to test anti-Hsc70/HSPA8 antibody PB9641 on rat colon carcinoma ovarian carcinoma for research purposes, then I may be interested in using anti-Hsc70/HSPA8 antibody PB9641 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

V. Parker

Verified customer

Asked: 2017-12-14

Answer

The products we sell, including anti-Hsc70/HSPA8 antibody PB9641, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-12-14

Question

Is a blocking peptide available for product anti-Hsc70/HSPA8 antibody (PB9641)?

Verified Customer

Verified customer

Asked: 2017-10-20

Answer

We do provide the blocking peptide for product anti-Hsc70/HSPA8 antibody (PB9641). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-10-20

Question

Is this PB9641 anti-Hsc70/HSPA8 antibody reactive to the isotypes of HSPA8?

Verified Customer

Verified customer

Asked: 2017-09-21

Answer

The immunogen of PB9641 anti-Hsc70/HSPA8 antibody is E.coli-derived human Hsc70 recombinant protein (Position: Q520-A614). Human Hsc70 shares 98.9% amino acid (aa) sequence identity with both mouse and rat Hsc70. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-09-21

Question

We ordered your anti-Hsc70/HSPA8 antibody for IHC on lymphoblast in the past. I am using rat, and I plan to use the antibody for WB next. We are interested in examining lymphoblast as well as frontal cortex in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

C. Dhar

Verified customer

Asked: 2015-08-21

Answer

I have checked the website and datasheets of our anti-Hsc70/HSPA8 antibody and it appears that PB9641 has been validated on rat in both IHC and WB. Thus PB9641 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2015-08-21

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon carcinoma ovarian carcinoma using anti-Hsc70/HSPA8 antibody PB9641. Let me know if you need anything else.

R. Parker

Verified customer

Asked: 2015-06-12

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-06-12

Question

My team were satisfied with the WB result of your anti-Hsc70/HSPA8 antibody. However we have seen positive staining in cervix carcinoma nucleolus. using this antibody. Is that expected? Could you tell me where is HSPA8 supposed to be expressed?

K. Dhar

Verified customer

Asked: 2014-07-03

Answer

Based on literature, cervix carcinoma does express HSPA8. Generally HSPA8 expresses in cytoplasm. melanosome. nucleus, nucleolus. Regarding which tissues have HSPA8 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1286667
Cervix carcinoma, Pubmed ID: 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Placenta, and Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2014-07-03

Conjugation

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PB9641
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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