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Product Info Summary
| SKU: | M01024 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Hsc70 HSPA8 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01024
BM4280 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Hsc70 HSPA8 Rabbit Monoclonal Antibody catalog # M01024. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hsc70 HSPA8 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01024)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DBB-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Hsc70
Reactive Species
M01024 is reactive to HSPA8 in Human, Mouse, Rat
Observed Molecular Weight
71 kDa
Calculated molecular weight
70.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01024 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:30
Positive Control
WB: human Hela whole cell, human 293T whole cell, human Caco-2 whole cell, human A549 whole cell, rat heart tissue, rat liver tissue, mouse heart tissue, mouse liver tissue
IHC: Rat ovary tissue, Human liver cancer tissue, Human breast cancer tissue, Rat liver tissue, Mouse lung tissue, human breast cancer tissue
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Rat ovary, using the Antibody at 1:200 dilution.
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Western blot analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (M01024).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSC70/HSPA8 antigen affinity purified monoclonal antibody (M01024) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSC70/HSPA8 at approximately 71 kDa. The expected band size for HSC70/HSPA8 is at 71 kDa.
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Immunohistochemical analysis of paraffin-embedded Human liver cancer, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human breast cancer, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat liver, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse lung, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using Hsc70 Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of MCF7 cells, using Hsc70 Antibody .
Specific Publications For Anti-Hsc70 HSPA8 Rabbit Monoclonal Antibody (M01024)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Hsc70 HSPA8 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-Hsc70 Rabbit Monoclonal antibody for WB for human lymphoblast on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lymphoblast identification?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
It shows on the product datasheet, M01024 anti-Hsc70 Rabbit Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lymphoblast in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-05
Question
I have a question about product M01024, anti-Hsc70 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Williams
Verified customer
Asked: 2020-04-16
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01024 anti-Hsc70 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-16
Question
See attached the WB image, lot number and protocol we used for lymphoblast using anti-Hsc70 Rabbit Monoclonal antibody M01024. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-05
Question
Our team were well pleased with the WB result of your anti-Hsc70 Rabbit Monoclonal antibody. However we have been able to see positive staining in cervix carcinoma erythroleukemia cytoplasm. melanosome. nucleus using this antibody. Is that expected? Could you tell me where is HSPA8 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
According to literature, cervix carcinoma erythroleukemia does express HSPA8. Generally HSPA8 expresses in cytoplasm. melanosome. nucleus, nucleolus. Regarding which tissues have HSPA8 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1286667
Cervix carcinoma, Pubmed ID: 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Placenta, and Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-17
Question
Do you have a BSA free version of anti-Hsc70 Rabbit Monoclonal antibody M01024 available?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Hsc70 Rabbit Monoclonal antibody M01024 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-29
Question
I see that the anti-Hsc70 Rabbit Monoclonal antibody M01024 works with WB, what is the protocol used to produce the result images on the product page?
S. Anderson
Verified customer
Asked: 2019-11-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-08
Question
Would M01024 anti-Hsc70 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
You can see on the product datasheet, M01024 anti-Hsc70 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-25
Question
Does anti-Hsc70 Rabbit Monoclonal antibody M01024 work for WB with lymphoblast?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
According to the expression profile of lymphoblast, HSPA8 is highly expressed in lymphoblast. So, it is likely that anti-Hsc70 Rabbit Monoclonal antibody M01024 will work for WB with lymphoblast.
Boster Scientific Support
Answered: 2019-10-22
Question
We have been able to see staining in human liver. What should we do? Is anti-Hsc70 Rabbit Monoclonal antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
According to literature liver does express HSPA8. According to Uniprot.org, HSPA8 is expressed in frontal cortex, placenta skin, embryonic kidney, colon carcinoma ovarian carcinoma, brain, cajal-retzius cell fetal brain cortex, lymphoblast, melanoma, leukemic t-cell, cervix carcinoma, cervix carcinoma erythroleukemia, liver, colon carcinoma, among other tissues. Regarding which tissues have HSPA8 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1286667
Cervix carcinoma, Pubmed ID: 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Placenta, and Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-12
Question
you antibody using your anti-Hsc70 Rabbit Monoclonal antibody for lysosome vesicle biogenesis studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
Thank you for your inquiry. This M01024 anti-Hsc70 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IP, IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-18
Question
We are currently using anti-Hsc70 Rabbit Monoclonal antibody M01024 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-03
Answer
The anti-Hsc70 Rabbit Monoclonal antibody (M01024) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-03
Question
I am interested in to test anti-Hsc70 Rabbit Monoclonal antibody M01024 on human lymphoblast for research purposes, then I may be interested in using anti-Hsc70 Rabbit Monoclonal antibody M01024 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-04-19
Answer
The products we sell, including anti-Hsc70 Rabbit Monoclonal antibody M01024, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-19
Question
Is a blocking peptide available for product anti-Hsc70 Rabbit Monoclonal antibody (M01024)?
Verified Customer
Verified customer
Asked: 2017-12-04
Answer
We do provide the blocking peptide for product anti-Hsc70 Rabbit Monoclonal antibody (M01024). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-12-04
Question
Is this M01024 anti-Hsc70 Rabbit Monoclonal antibody reactive to the isotypes of HSPA8?
Verified Customer
Verified customer
Asked: 2017-09-07
Answer
The immunogen of M01024 anti-Hsc70 Rabbit Monoclonal antibody is A synthesized peptide derived from human Hsc70 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-07
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymphoblast using anti-Hsc70 Rabbit Monoclonal antibody M01024. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-06
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-06
Question
We ordered your anti-Hsc70 Rabbit Monoclonal antibody for IF on frontal cortex in the past. I am using human, and We are going to use the antibody for IP next. We are interested in examining frontal cortex as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
A. Miller
Verified customer
Asked: 2017-03-22
Answer
I have checked the website and datasheets of our anti-Hsc70 Rabbit Monoclonal antibody and it seems that M01024 has been validated on human in both IF and IP. Thus M01024 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-03-22
