Anti-HSD17B1 Antibody

Product Name

Anti-HSD17B1 Antibody

View all 17 beta-HSD1/HSD17B1 Antibodies

SKU/Catalog Number

PA1613-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HSD17B1 Antibody catalog # PA1613-1. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HSD17B1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1613-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human HSD17B1, different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1613-1 is reactive to HSD17B1 in Human, Rat

Applications

PA1613-1 is guaranteed for IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

37-38 kDa

Calculated molecular weight

34.95kDa

Background of 17 beta-HSD1/HSD17B1

Estradiol 17-beta-dehydrogenase 1 is an enzyme that in humans is encoded by the HSD17B1 gene. This gene encodes a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. The encoded protein catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. This gene is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Polymorphisms in this gene have been linked to breast and prostate cancer. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of HSD17B1 using anti-HSD17B1 antibody (PA1613-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human T47D whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B1 antigen affinity purified polyclonal antibody (Catalog # PA1613-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B1 at approximately 37-38 kDa. The expected band size for HSD17B1 is at 35 kDa.

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Figure 2. IHC analysis of HSD17B1 using anti-HSD17B1 antibody (PA1613-1).
HSD17B1 was detected in a paraffin-embedded section of Human Placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HSD17B1 Antibody (PA1613-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 3. IHC analysis of HSD17B1 using anti-HSD17B1 antibody (PA1613-1).
HSD17B1 was detected in a paraffin-embedded section of Rat Liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HSD17B1 Antibody (PA1613-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 4. IHC analysis of HSD17B1 using anti-HSD17B1 antibody (PA1613-1).
HSD17B1 was detected in a paraffin-embedded section of Rat Ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HSD17B1 Antibody (PA1613-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Click image to see more details

Figure 5. ICC analysis of HSD17B1 using anti-HSD17B1 antibody (PA1613-1).
HSD17B1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-HSD17B1 Antibody (PA1613-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Gene/Protein Information For HSD17B1 (Source: Uniprot.org, NCBI)

Alternative Names

17 betaHSD1; 17 beta-HSD1; 17-beta-HSD 1; 17-beta-hydroxysteroid dehydrogenase type 1; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; E17KSR; E2DH; EC 1.1.1.62; EDH17B1; EDH17B2; EDH17B2EDHB17; EDHB17; estradiol 17-beta-dehydrogenase 1; estradiol 17-beta-dehydrogenase-1; HSD17; HSD17B1; hydroxysteroid (17-beta) dehydrogenase 1 isoform; hydroxysteroid (17-beta) dehydrogenase 1; MGC138140; Placental 17-beta-hydroxysteroid dehydrogenase; SDR28C1; short chain dehydrogenase/reductase family 28CE, member 1 HSD17B1 17-beta-HSD, 20-alpha-HSD, E2DH, EDH17B2, EDHB17, HSD17, SDR28C1 hydroxysteroid 17-beta dehydrogenase 1 17-beta-hydroxysteroid dehydrogenase type 1|17-beta-HSD 1|20 alpha-hydroxysteroid dehydrogenase|estradiol 17-beta-dehydrogenase 1|placental 17-beta-hydroxysteroid dehydrogenase|short chain dehydrogenase/reductase family 28C member 1|short chain dehydrogenase/reductase family 28CE, member 1

For more info on HSD17B1, check out the HSD17B1 Infographic

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In this infographic, you will see the following information for HSD17B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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