Anti-HSD17B8 Antibody Picoband™

Product Name

Anti-HSD17B8 Antibody Picoband™

View all HSD17B8 Antibodies

SKU/Catalog Number

A08741-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HSD17B8 Antibody Picoband™ catalog # A08741-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-HSD17B8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08741-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human HSD17B8 recombinant protein (Position: D42-D244).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A08741-1 is reactive to HSD17B8 in Human, Mouse, Rat

Applications

A08741-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

34 kDa

Calculated molecular weight

26.974kDa

Background of HSD17B8

Estradiol 17 beta-dehydrogenase 8 is an enzyme that in humans is encoded by the HSD17B8 gene. In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected, but the full-length nature of this variant has not been determined.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of HSD17B8 using anti-HSD17B8 antibody (A08741-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B8 antigen affinity purified polyclonal antibody (Catalog # A08741-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B8 at approximately 34 kDa. The expected band size for HSD17B8 is at 34 kDa.

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Figure 2. Flow Cytometry analysis of JK cells using anti-HSD17B8 antibody (A08741-1).
Overlay histogram showing JK cells stained with A08741-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSD17B8 Antibody (A08741-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For HSD17B8 (Source: Uniprot.org, NCBI)

Alternative Names

D6S2245EdJ1033B10.9; EC 1.1.1.-; EC 1.1.1.62; EC 1.1.1.63,17-beta-HSD 8; estrogen 17-oxidoreductase; FABGLestradiol 17-beta-dehydrogenase 8; H2-KE6; HKE6FabG (beta-ketoacyl-[acyl-carrier-protein] reductase, E coli) like (E. coli); hydroxysteroid (17-beta) dehydrogenase 8,3-oxoacyl-[acyl-carrier-protein] reductase; KE6; ke-6; Ke-6,17-beta-hydroxysteroid dehydrogenase 8; Protein Ke6; Really interesting new gene 2 protein; RING2FABG; SDR30C1; short chain dehydrogenase/reductase family 30C, member 1; Testosterone 17-beta-dehydrogenase 8 HSD17B8 D6S2245E, FABG, FABGL, H2-KE6, HKE6, KE6, RING2, SDR30C1, dJ1033B10.9 hydroxysteroid 17-beta dehydrogenase 8 estradiol 17-beta-dehydrogenase 8|17-beta-HSD 8|17-beta-hydroxysteroid dehydrogenase 8|3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit|3-oxoacyl-[acyl-carrier-protein] reductase|KAR alpha subunit|estrogen 17-oxidoreductase|ke-6|protein Ke6|really interesting new gene 2 protein|short chain dehydrogenase/reductase family 30C member 1|testosterone 17-beta-dehydrogenase 8

For more info on HSD17B8, check out the HSD17B8 Infographic

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