Anti-HSF1 Monoclonal Antibody

Product Name

Anti-HSF1 Monoclonal Antibody

View all HSF1 Antibodies

SKU/Catalog Number

M00250

Size

100ug

Form

liquid

Description

Boster Bio Anti-HSF1 Monoclonal Antibody catalog # M00250. Tested in ELISA, IP, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HSF1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00250)

Host

Rat

Contents

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

10H8

Isotype

IgG1

Immunogen

Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~85kDa (unstressed cell lysates), and~95kDa (heat shocked cell lysates).

Reactive Species

M00250 is reactive to HSF1 in Bovine, Guinea pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat

Applications

M00250 is guaranteed for ELISA, IP, IF, IHC, ICC, WB, Gel Shift Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

57.26kDa

Background of HSF1

HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB (1:1000), IHC (1:1000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

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Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (M00250) . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (M00250) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (M00250) . Tissue: Heat Shocked mitotic HeLa cells. Species: Human. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (M00250) at 1:1000. HSF1 stained green. Courtesy of: Morimoto Lab, Northwestern University, USA.

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Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (M00250) . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (M00250) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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Immunohistochemistry analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (M00250). Tissue: Breast carcinoma. Species: Human. Fixation: 10% Formalin Solution for 20 hours at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (M00250) at 1:1000 for 40 min. Secondary Antibody: Dako labeled Polymer HRP Anti-rat IgG, DAB Chromogen (brown) (Dako Envision+ System) for 30 min at RT. Counterstain: Mayer’s Hematoxylin (purple/blue) nuclear stain for 1 minute at RT. Localization: Nuclear. Magnification: 100X. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.

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Western Blot analysis of Human Breast adenocarcinoma cell line (MCF7) showing detection of ~65 kDa HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (M00250). Lane 1: MW ladder. Lane 2: HSF1 null lysate prepared from mouse embryonic fibroblasts. Lane 3: MCF7 lysate (5 µg). Lane 4: MCF7 lysate (10 µg). Lane 5: MCF7 lysate (20 µg). Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (M00250) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rat IgG: HRP for 1 hour at RT. Predicted/Observed Size: ~65 kDa. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.

Gene/Protein Information For HSF1 (Source: Uniprot.org, NCBI)

Alternative Names

heat shock factor protein 1; heat shock transcription factor 1HSTF1HSF 1; HSF1; HSTF 1; HSTF1 HSF1 HSTF1 heat shock transcription factor 1 heat shock factor protein 1

For more info on HSF1, check out the HSF1 Infographic

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In this infographic, you will see the following information for HSF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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