Anti-Hsp40/DNAJB1 Antibody

Product Name

Anti-Hsp40/DNAJB1 Antibody

View all HSP40/DNAJB1 Antibodies

SKU/Catalog Number

PA1043

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Hsp40/DNAJB1 Antibody catalog # PA1043. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Hsp40/DNAJB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1043)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp40 (317-332aa EFEVIFPERIPQTSRT), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1043 is reactive to DNAJB1 in Human, Mouse, Rat

Applications

PA1043 is guaranteed for IHC, WB Boster Guarantee

Background of HSP40/DNAJB1

The Hsp40 (heat shock protein with molecular size of approximately 40 kDa) is one of the mammalian homologues of bacterial DnaJ heat shock protein. Ohtsuka (1993) isolated a cDNA encoding a 40-kD heat-shock protein designated HSPF1. The deduced 340-amino acid HSPF1 protein is 34% identical to E. coli DnaJ and 34% and 36% identical to HSJ1 and HSJ2, respectively. HSPF1 gene spans over 7 kb and contains 3 exons and 2 introns. HSPF1 gene is mapped to chromosome 19p13.2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

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Anti-Hsp40 antibody, PA1043, Western blotting
WB: HELA Cell Lysate

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Anti-Hsp40 antibody, PA1043, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Gene/Protein Information For DNAJB1 (Source: Uniprot.Org, NCBI)

Alternative Names

DnaJ (Hsp40) homolog, subfamily B, member 1; DnaJ (Hsp40) homolog, subfmaily B, member 1; dnaJ homolog subfamily B member 1; DnaJ protein homolog 1; DNAJ1; DNAJB1; Hdj1; hDj-1; Heat shock 40 kDa protein 1; heat shock 40kD protein 1; Heat shock protein 40; HSP40; HSPF1; HSPF1Hdj1; Human DnaJ protein 1; radial spoke 16 homolog B; RSPH16B; Sis1 DNAJB1 HSPF1, Hdj1, Hsp40, RSPH16B, Sis1 DnaJ heat shock protein family (Hsp40) member B1 dnaJ homolog subfamily B member 1|DnaJ (Hsp40) homolog, subfamily B, member 1|dnaJ protein homolog 1|heat shock 40 kDa protein 1|human DnaJ protein 1|radial spoke 16 homolog B

For more info on DNAJB1, check out the DNAJB1 Infographic

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In this infographic you will see the following information for DNAJB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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