Anti-Hsp40/DNAJB1 Antibody

HSP40/DNAJB1 antibody

Boster Bio Anti-Hsp40/DNAJB1 Antibody catalog # PA1043. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1043
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Hsp40/DNAJB1 Antibody

View all HSP40/DNAJB1 Antibodies

SKU/Catalog Number







Boster Bio Anti-Hsp40/DNAJB1 Antibody catalog # PA1043. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Hsp40/DNAJB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1043)




Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.




A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp40 (317-332aa EFEVIFPERIPQTSRT), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1043 is reactive to DNAJB1 in Human, Mouse, Rat


PA1043 is guaranteed for IHC, WB Boster Guarantee

Background of HSP40/DNAJB1

The Hsp40 (heat shock protein with molecular size of approximately 40 kDa) is one of the mammalian homologues of bacterial DnaJ heat shock protein. Ohtsuka (1993) isolated a cDNA encoding a 40-kD heat-shock protein designated HSPF1. The deduced 340-amino acid HSPF1 protein is 34% identical to E. coli DnaJ and 34% and 36% identical to HSJ1 and HSJ2, respectively. HSPF1 gene spans over 7 kb and contains 3 exons and 2 introns. HSPF1 gene is mapped to chromosome 19p13.2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For DNAJB1 (Source: Uniprot.Org, NCBI)

Uniprot ID


Gene ID


Gene Name


Full Name

DnaJ homolog subfamily B member 1



Alternative Names

DnaJ (Hsp40) homolog, subfamily B, member 1; DnaJ (Hsp40) homolog, subfmaily B, member 1; dnaJ homolog subfamily B member 1; DnaJ protein homolog 1; DNAJ1; DNAJB1; Hdj1; hDj-1; Heat shock 40 kDa protein 1; heat shock 40kD protein 1; Heat shock protein 40; HSP40; HSPF1; HSPF1Hdj1; Human DnaJ protein 1; radial spoke 16 homolog B; RSPH16B; Sis1 DNAJB1 HSPF1, Hdj1, Hsp40, RSPH16B, Sis1 DnaJ heat shock protein family (Hsp40) member B1 dnaJ homolog subfamily B member 1|DnaJ (Hsp40) homolog, subfamily B, member 1|dnaJ protein homolog 1|heat shock 40 kDa protein 1|human DnaJ protein 1|radial spoke 16 homolog B

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on DNAJB1, check out the DNAJB1 Infographic

DNAJB1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for DNAJB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for PA1043

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5 Customer Q&As for Anti-Hsp40/DNAJB1 Antibody


I am looking for to test anti-Hsp40/DNAJB1 antibody PA1043 on rat testis for research purposes, then I may be interested in using anti-Hsp40/DNAJB1 antibody PA1043 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-03-17


The products we sell, including anti-Hsp40/DNAJB1 antibody PA1043, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-03-17


I see that the anti-Hsp40/DNAJB1 antibody PA1043 works with IHC, what is the protocol used to produce the result images on the product page?

K. Patel

Verified customer

Asked: 2019-09-27


You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-27


Would PA1043 anti-Hsp40/DNAJB1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

G. Lewis

Verified customer

Asked: 2019-04-22


You can see on the product datasheet, PA1043 anti-Hsp40/DNAJB1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-04-22


We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-Hsp40/DNAJB1 antibody PA1043. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-07-04


Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-07-04


We are currently using anti-Hsp40/DNAJB1 antibody PA1043 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?

K. Mangal

Verified customer

Asked: 2017-06-21


The anti-Hsp40/DNAJB1 antibody (PA1043) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-06-21


Total: $315


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