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- Table of Contents
ITPR1 antibody (PB9225) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Flow Cytometry analysis of U87 cells using anti-IP3 receptor antibody (PB9225).
Overlay histogram showing U87 cells stained with PB9225 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IP3 receptor Antibody (PB9225,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U87 cells stained with PB9225 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IP3 receptor Antibody (PB9225,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-IP3 receptor/ITPR1 Antibody Picoband®
ITPR1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
ITPR1 antibody was positively detected by WB in:
human Hela whole cell, rat brain tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ITPR1 antibody was positively detected by IHC in:
Mouse Brain tissue, Rat Brain tissue, Human Lung Cancer tissue, human brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ITPR1 antibody was positively detected by ICC/IF in:
U20S cell
ITPR1 antibody was positively detected by FC in:
U87 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-IP3 receptor/ITPR1 Antibody Picoband®
View all ITPR1 Antibodies
View all ITPR1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human IP3 receptor recombinant protein (Position: A2411-A2758). Human IP3 receptor shares 98% and 97% amino acid (aa) sequences identity with mouse and rat IP3 receptor, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
290 kDa
Calculated Molecular Weight
313.9 kDa
Background of ITPR1
Inositol 1,4,5-trisphosphate receptor type 1, also known as IP3R or IP3R1, is a protein that in humans is encoded by the ITPR1 gene. It is mapped to 3p26.1. The product of the ITPR1 gene is predominantly enriched in cerebellar Purkinje cells but is also concentrated in neurons in the hippocampal CA1 region, caudate-putamen, and cerebral cortex. The ITPR1 gene encodes the inositol 1,4,5-triphosphate (IP3) receptor, an intracellular IP3-gated calcium channel that modulates intracellular calcium signaling. Upon stimulation by inositol 1,4,5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in ITPR1 cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders.
Cite This Product
Anti-IP3 receptor/ITPR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9225)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Dog
98%
High Homology
Mouse
98%
High Homology
Sheep
98%
High Homology
Rat
97%
High Homology
Cow
97%
High Homology
Rabbit
95%
High Homology
Zebrafish
83%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ITPR1 antibody
Immunohistochemistry (IHC) protocol for ITPR1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ITPR1 antibody
Flow Cytometry (FC) protocol for ITPR1 antibody
Publications
Application:
Species:
Selenoprotein K-dependent MyD88 palmitoylation promotes hepatic metaflammation in high-fat diet fed mice
Published: 2025-05-21
Journal: FREE RADICAL BIOLOGY AND MEDICINE
Application: WB
Impact Factor: 8.20
Species: Mouse
PMID: 40409695
LncRNA LINC00662 Exerts an Oncogenic Effect on Osteosarcoma by the miR-16-5p/ITPR1 Axis
Published: 2021-09-28
Journal: Journal of Oncology
Application: WB
Impact Factor: 4.38
Species: Human
PMID: 34621314
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Product Promise
We promise Anti-IP3 receptor/ITPR1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9225
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026