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Product Info Summary
| SKU: | M01103-3 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-Hsp90 alpha HSP90AA1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01103-3
BM4067 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Hsp90 alpha HSP90AA1 Rabbit Monoclonal Antibody catalog # M01103-3. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hsp90 alpha HSP90AA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01103-3)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BAC-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Hsp90 alpha
Reactive Species
M01103-3 is reactive to HSP90AA1 in Human, Mouse, Rat
Observed Molecular Weight
85 kDa
Calculated molecular weight
84.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01103-3 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: PC-12 cell, HeLa cell
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of Hsp90 alpha expression in (1)PC-12 cell lysate; (2)HeLa cell lysate.
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Immunofluorescent analysis of Hela cells, using Hsp90 alpha Antibody .
Specific Publications For Anti-Hsp90 alpha HSP90AA1 Rabbit Monoclonal Antibody (M01103-3)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Hsp90 alpha HSP90AA1 Rabbit Monoclonal Antibody
Question
My boss were content with the WB result of your anti-Hsp90 alpha Rabbit Monoclonal antibody. However we have observed positive staining in cervix carcinoma nucleus. using this antibody. Is that expected? Could you tell me where is HSP90AA1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
According to literature, cervix carcinoma does express HSP90AA1. Generally HSP90AA1 expresses in nucleus. Regarding which tissues have HSP90AA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 2492519, 10409742
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 18318008, 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Peripheral blood lymphocyte, Pubmed ID: 2780322
Pituitary, Pubmed ID: 16807684
Placenta, Pubmed ID: 2527334, 15489334
Placenta, and Teratocarcinoma, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Renal cell carcinoma, Pubmed ID: 10508479, 11274138
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2020-03-03
Question
We have observed staining in rat placenta. What should we do? Is anti-Hsp90 alpha Rabbit Monoclonal antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2018-10-12
Answer
Based on literature placenta does express HSP90AA1. Based on Uniprot.org, HSP90AA1 is expressed in middle temporal gyrus, peripheral blood lymphocyte, placenta, placenta teratocarcinoma, brain, cajal-retzius cell fetal brain cortex, heart, renal cell carcinoma, lymphoblast, cervix carcinoma, pituitary, t-cell, platelet, liver, leukemic t-cell, melanoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have HSP90AA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 2492519, 10409742
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 18318008, 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Peripheral blood lymphocyte, Pubmed ID: 2780322
Pituitary, Pubmed ID: 16807684
Placenta, Pubmed ID: 2527334, 15489334
Placenta, and Teratocarcinoma, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Renal cell carcinoma, Pubmed ID: 10508479, 11274138
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2018-10-12
Question
We are currently using anti-Hsp90 alpha Rabbit Monoclonal antibody M01103-3 for rat tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
D. Miller
Verified customer
Asked: 2016-01-07
Answer
The anti-Hsp90 alpha Rabbit Monoclonal antibody (M01103-3) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-01-07
Question
Our lab used your anti-Hsp90 alpha Rabbit Monoclonal antibody for IF on cajal-retzius cell fetal brain cortex in a previous project. I am using rat, and We intend to use the antibody for IP next. We are interested in examining cajal-retzius cell fetal brain cortex as well as renal cell carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for IP?
E. Krishna
Verified customer
Asked: 2013-09-11
Answer
I viewed the website and datasheets of our anti-Hsp90 alpha Rabbit Monoclonal antibody and I see that M01103-3 has been tested on rat in both IF and IP. Thus M01103-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-09-11
