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Product Info Summary
| SKU: | M04307 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-F, WB |
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Product info
Product Name
Anti-Hu CD123 Purified IL3RA Monoclonal Antibody
SKU/Catalog Number
M04307
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD123 Purified IL3RA Monoclonal Antibody (Catalog# M04307). Tested in Flow Cytometry, IP, WB, IHC-F application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD123 Purified IL3RA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04307)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
6H6
Isotype
Mouse IgG1
Immunogen
IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells. The mouse monoclonal antibody 6H6 recognizes an extracellular epitope of CD123 (interleukin 3 receptor alpha), a 60-70 kDa transmembrane protein expressed by myeloid precursors, megakaryocytes, macrophages, dendritic cells, mast cells, basophils, and some B cells. This antibody does not inhibit IL-3 binding to its receptor.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M04307 is reactive to IL3RA in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
43.3 kDa
Background of IL3RA
CD123 is the alpha chain of interleukin 3 receptor (IL-3R alpha). This subunit heterodimerizes with the interleukin 3 receptor beta chain (CD131), which is shared with other receptors. CD123 interacts with IL-3 specifically, but with low affinity, and association with the beta subunit confers high affinity binding to the receptor heterodimer. Both chains are required for signaling, but receptor activation and signal transduction depend on IL-3 binding to CD123 as the initial step.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04307 is guaranteed for Flow Cytometry, IP, IHC-F, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Validation Images & Assay Conditions
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Separation of CD123 positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD123 (6H6) purified antibody (concentration in sample 0,11 µg/ml, GAM APC).
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Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD123 (6H6) purified antibody (concentration in sample 0,11 µg/ml, GAM APC).
Specific Publications For Anti-Hu CD123 Purified IL3RA Monoclonal Antibody (M04307)
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