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Product Info Summary
| SKU: | M03988 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, ICC |
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Product info
Product Name
Anti-Hu CD150 Purified Azide Free SLAMF1 Monoclonal Antibody
SKU/Catalog Number
M03988
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD150 Purified Azide Free SLAMF1 Monoclonal Antibody (Catalog# M03988). Tested in Flow Cytometry, IP, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD150 Purified Azide Free SLAMF1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03988)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, azide-free
Clonality
Monoclonal
Clone Number
SLAM.4
Isotype
Mouse IgG1
Immunogen
Human CD150-transfected 300.19 cells. The mouse monoclonal antibody SLAM.4 recognizes an extracellular epitope of CD150, a cell surface molecule expressed on lymphocytes and involved in their activation.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M03988 is reactive to SLAMF1 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
37.2 kDa
Background of SLAMF1
CD150, also known as SLAM (signaling lymphocyte activation molecule) is a 70-95 kDa single chain transmembrane phosphoglycoprotein of the CD2 family. Its extracellular part contains eight potential N-glycosylation sites, and the intracellular tail contains three unique tyrosine-based motifs. These binding sites can be recognized by SH2-binding phosphatases and the adaptor proteins, such as SAP/SH2D1A or EAT-2. The SLAM family receptors are involved in leucocyte activation and contribute to the effective germinal center formation, generation of high-affinity antibody-secreting plasma cells, and memory T and B cells, thereby facilitating long-term immune response. CD150 expression is upregulated after cell activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03988 is guaranteed for Flow Cytometry, IP, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-5 μg/ml.
Validation Images & Assay Conditions
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Separation of human CD150 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD150 (SLAM.4) purified antibody (concentration in sample 5 µg/ml, GAM APC).
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Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD150 (SLAM.4) purified antibody (concentration in sample 5 µg/ml, GAM APC).
Specific Publications For Anti-Hu CD150 Purified Azide Free SLAMF1 Monoclonal Antibody (M03988)
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