Anti-Hu CD235a Purified GYPA Monoclonal Antibody

Product Name

Anti-Hu CD235a Purified GYPA Monoclonal Antibody

View all Glycophorin A Antibodies

SKU/Catalog Number

M02184-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD235a Purified GYPA Monoclonal Antibody (Catalog# M02184-2). Tested in Flow Cytometry, IHC-P, IHC-F application(s). This antibody reacts with Rat, Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD235a Purified GYPA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02184-2)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

JC159

Isotype

Mouse IgG1

Immunogen

Membrane preparation from splenic hairy cell leukemia. The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M02184-2 is reactive to GYPA in Human, Rat

Applications

M02184-2 is guaranteed for Flow Cytometry, IHC-P, IHC-F Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

16.331kDa

Background of Glycophorin A

CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (paraffin sections): 10 μg/ml.
Flow cytometry: 1-4 µg/ml

Validation Images & Assay Conditions

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Figure 1. Flow Cytometry validation of GYPA using Anti-Hu CD235a Purified GYPA Monoclonal Antibody (M02184-2).

Flow cytometry analysis (surface staining) of CD235a in human peripheral blood (erythrocytes and leukocytes) with anti-CD235a (JC159) purified

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Figure 2. Immunohistochemistry validation of GYPA using Anti-Hu CD235a Purified GYPA Monoclonal Antibody (M02184-2).

Immunohistochemistry staining of human adrenal (paraffin-embedded sections) with anti-CD235a (JC159)

Gene/Protein Information For GYPA (Source: Uniprot.org, NCBI)

Alternative Names

CD235a antigen; CD235a; glycophorin A (includes MN blood group); glycophorin A (MNS blood group); Glycophorin A; glycophorin Erik; glycophorin MiI; glycophorin MiIII; glycophorin MiV; glycophorin MiX; glycophorin SAT; glycophorin Sta type C; glycophorin-A; GPA; GPErik; GpMiIII; GPSAT; GYPA; HGpMiIII; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); Mi.V glycoprotein (24 AA); MN sialoglycoprotein; MNS; PAS-2; recombinant glycophorin A-B Miltenberger-DR GYPA CD235a, GPA, GPErik, GPSAT, HGpMiV, HGpMiXI, HGpSta(C), MN, MNS, PAS-2 glycophorin A (MNS blood group) glycophorin-A|MN sialoglycoprotein|Mi.V glycoprotein (24 AA)|erythroid-lineage-specific membrane sialoglycoprotein|glycophorin A (MN blood group)|glycophorin A, GPA|glycophorin Erik|glycophorin MiI|glycophorin MiV|glycophorin SAT|glycophorin Sta type C|recombinant glycophorin A-B Miltenberger-DR|sialoglycoprotein alpha

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In this infographic, you will see the following information for GYPA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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