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Product Info Summary
| SKU: | M00914-3 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-P, WB |
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Product info
Product Name
Anti-Hu CD59 Purified Monoclonal Antibody
SKU/Catalog Number
M00914-3
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD59 Purified Monoclonal Antibody (Catalog# M00914-3). Tested in WB, Flow Cytometry, IP, IHC-P application(s). This antibody reacts with Mouse, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD59 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00914-3)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
MEM-43/5
Isotype
Mouse IgG2b
Immunogen
Thymocytes and T lymphocytes. The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored extracellular glycoprotein expressed on the surface of all hematopoietic cells; it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies.
Reactive Species
M00914-3 is reactive to CD59 in Human, Mouse
Observed Molecular Weight
42 kDa
Calculated molecular weight
14.2 kDa
Background of CD59
CD59 (protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00914-3 is guaranteed for Flow Cytometry, IP, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 0.5-4 μg/ml; positive control: blood.
Immunohistochemistry (paraffin sections): 5 μg/ml.
Western blotting: 1-2 μg/ml; positive control: mouse spleen, non-reducing conditions.
Validation Images & Assay Conditions
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Western blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5).
Lane 1: original cell lysate
Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43)
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Separation of human neutrophil granulocytes (red-filled) from human CD59 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD59 (MEM-43/5) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).
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Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD59 (MEM-43/5) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).
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Immunohistochemistry staining of human skin (paraffin sections) using anti-CD59 (MEM-43/5). Commercially tested by LifeSpan BioSciences.
Specific Publications For Anti-Hu CD59 Purified Monoclonal Antibody (M00914-3)
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