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Product Info Summary
| SKU: | M02300-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-P, ICC, WB |
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Product info
Product Name
Anti-Hu CD82 Purified Monoclonal Antibody
SKU/Catalog Number
M02300-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD82 Purified Monoclonal Antibody (Catalog# M02300-1). Tested in Flow Cytometry, IP, WB, IHC-P, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD82 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02300-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
C33
Isotype
Mouse IgG2a
Immunogen
C91/PL (human HTLV-1+ T cell line). The mouse monoclonal antibody C33 recognizes an extracellular/luminal epitope of CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M02300-1 is reactive to CD82 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
29.6 kDa
Background of CD82
CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02300-1 is guaranteed for Flow Cytometry, IP, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 µg/ml
Validation Images & Assay Conditions
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Separation of human CD82 positive lymphocytes (red-filled) from CD82 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD82 (C33) purified antibody (concentration in sample 1 µg/ml) GAM APC.
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Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD82 (C33) purified antibody (concentration in sample 1 µg/ml) GAM APC.
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Immunohistochemistry staining of human tonsil (paraffin-embedded sections) with anti-CD82 (C33). Commercially tested by LifeSpan BioSciences.
Specific Publications For Anti-Hu CD82 Purified Monoclonal Antibody (M02300-1)
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