Anti-Hu CD85j Purified LILRB1 Monoclonal Antibody

Product Name

Anti-Hu CD85j Purified LILRB1 Monoclonal Antibody

View all LILRB1 Antibodies

SKU/Catalog Number

M03208

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD85j Purified LILRB1 Monoclonal Antibody (Catalog# M03208). Tested in Flow Cytometry, IP, WB application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD85j Purified LILRB1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03208)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

GHI/75

Isotype

Mouse IgG2b kappa

Immunogen

Hairy cell leukaemia cells. The mouse monoclonal antibody GHI/75 recognizes an extracellular epitope of CD85j / ILT2, an 110-120 kDa membrane glycoprotein expressed strongly on plasma cells, moderately on circulating B cells, and weakly on monocytes. It is also expressed on T cell and NK cell subsets (variable, individual).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M03208 is reactive to LILRB1 in Human

Applications

M03208 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

70.819kDa

Background of LILRB1

CD85j, also known as ILT-2 (Ig-like transcript 2), LIR-1 (leukocyte Ig-like receptor 1), or LILRB1 (leukocyte Ig-like receptor B1), is a member of Ig superfamily transmembrane glycoproteins named CD85. The CD85j protein is expressed on several types of immune cells (plasma cells, B cells, monocytes, T and NK cell subsets) where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 μg/ml.

Validation Images & Assay Conditions

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Figure 1. Flow Cytometry validation of LILRB1 using Anti-Hu CD85j Purified LILRB1 Monoclonal Antibody (M03208).

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD85j(GHI/75) purified antibody (concentration in sample 1 μg/ml) GAM APC.

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Figure 2. Flow Cytometry validation of LILRB1 using Anti-Hu CD85j Purified LILRB1 Monoclonal Antibody (M03208).

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD85j (GHI/75) purified antibody (concentration in sample 1 μg/ml) GAM APC and anti-human CD19 (LT19) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

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Figure 3. Flow Cytometry validation of LILRB1 using Anti-Hu CD85j Purified LILRB1 Monoclonal Antibody (M03208).

Separation of human CD85j positive B cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD85j(GHI/75) purified antibody (concentration in sample 1 μg/ml) GAM APC.

Gene/Protein Information For LILRB1 (Source: Uniprot.org, NCBI)

Alternative Names

CD85 antigen-like family member J; CD85j antigen; CD85j; Ig-like transcript 2; ILT2; ILT-2; ILT2FLJ37515; Immunoglobulin-like transcript 2; leukocyte Ig-like receptor-1; Leukocyte immunoglobulin-like receptor 1; leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform; leukocyte immunoglobulin-like receptor subfamily B member 1; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB1; LIR1; LIR1LIR-1MIR-7CD85MIR7CD85 antigen; member 1; MIR7; Monocyte/macrophage immunoglobulin-like receptor 7 LILRB1 CD85J, ILT-2, ILT2, LIR-1, LIR1, MIR-7, MIR7, PIR-B, PIRB leukocyte immunoglobulin like receptor B1 leukocyte immunoglobulin-like receptor subfamily B member 1|CD85 antigen-like family member J|Ig-like transcript 2|leucocyte Ig-like receptor B1|leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1|monocyte/macrophage immunoglobulin-like receptor 7|myeloid inhibitory receptor 7

For more info on LILRB1, check out the LILRB1 Infographic

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In this infographic, you will see the following information for LILRB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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