Human IL1R2/Il 1 Rii ELISA Kit PicoKine™

Human IL1R2/Il 1 Rii ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL1R2 in cell culture supernatants and serum. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0396
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants and serum.

Product Name

Human IL1R2/Il 1 Rii ELISA Kit PicoKine™

See all IL-1 RII products

SKU/Catalog Number

EK0396

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL1R2/Il 1 Rii ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL1R2 in cell culture supernatants and serum. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human IL1R2/Il 1 Rii ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0396)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: sf21; Immunogen sequence: F14-E343

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0396 is reactive to IL1R2 in Human samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK0396 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0396-CAP Anti-Human IL1R2 Pre-coated 96-well strip microplate 1
EK0396-ST Human IL1R2 Standard 2 vials, 10 ng/tube
EK0396-DA Human IL1R2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)02000200020002000200020002000
O.D.0.0660.0880.1440.2540.5441.0071.8212.388

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL1R2/Il 1 Rii ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)10026183899279861
Standard deviation7.914.6142.737.9217.8557.68
CV (%)7.9%5.6%6.7%%8%6.4%6.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-1 RII in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1100948986925.35.7%
Sample 226129129327427913.14.6%
Sample 383880484784883417.892.1%
*number of samples for each test n=16.

Gene/Protein Information For IL1R2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P27930

Gene ID

7850

Gene Name

IL1R2

Full Name

Interleukin-1 receptor type 2

Weight

45.421kDa

Superfamily

interleukin-1 receptor family

Alternative Names

beta; CD121b antigen; CD121b; IL-1 R beta; IL-1 RII; IL1R2; IL1RBCD121 antigen-like family member B; IL-1R-beta; IL1RII; IL-1RII; IL-1RT2; IL-1RT-2; interleukin 1 receptor, type II; Interleukin-1 receptor beta; MGC47725 IL1R2 CD121b, CDw121b, IL-1R-2, IL-1RT-2, IL-1RT2c, IL1RB, IL1R2 interleukin 1 receptor type 2 interleukin-1 receptor type 2|CD121 antigen-like family member B|IL-1 type II receptor|IL-1R-beta|antigen CDw121b|interleukin 1 receptor type II variant 3|interleukin-1 receptor beta|interleukin-1 receptor type II|type II interleukin-1 receptor, beta

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL1R2, check out the IL1R2 Infographic

IL1R2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL1R2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Human IL1R2/Il 1 Rii ELISA Kit PicoKine™

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IL-1 RII using the EK0396 Human IL-1 RII Picokine® ELISA Kit?

W. Rodriguez

Verified customer

Asked: 2020-10-16

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2020-10-16

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-10-03

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-10-03

Question

Q: how to thaw whole blood sample for IL-1 RII ELISA after freezing?

B. Scott

Verified customer

Asked: 2019-10-01

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-1 RII for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-10-01

Question

Q: Can IL-1 RII ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2017-09-29

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2017-09-29

Question

Q: if the enzyme conjugated IL-1 RII antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-1 RII antigen?

Verified Customer

Verified customer

Asked: 2016-12-01

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-12-01

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