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Product Info Summary
| SKU: | A30608 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HUCE1 Antibody
SKU/Catalog Number
A30608
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HUCE1 Antibody (Catalog# A30608). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HUCE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30608)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human HUCE1. Immunogen sequence location: 271-320
Reactive Species
A30608 is reactive to RRP8 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
50.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30608 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western Blot analysis of MCF-7 cells using Cerebral 1 Polyclonal Antibody.
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Western blot analysis of lysates from MCF-7 cells, using HUCE1 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-HUCE1 Antibody (A30608)
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1 Customer Q&As for Anti-HUCE1 Antibody
Question
We are currently using anti-HUCE1 antibody A30608 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
B. Parker
Verified customer
Asked: 2014-12-15
Answer
The anti-HUCE1 antibody (A30608) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-12-15
