Anti-human CD4 Monoclonal Antibody Unconjugated, Flow Validated

Product Name

Anti-human CD4 Monoclonal Antibody Unconjugated, Flow Validated

View all CD4 Antibodies

SKU/Catalog Number

FC00344

Size

100ug, 500ug

Form

Liquid

Description

Boster Bio Anti-human CD4 Monoclonal Antibody Unconjugated, Flow Validated (Catalog# FC00344). Tested in Flow Cytometry application(s). This antibody reacts with Human, Rhesus monkey, Cynomolgus monkey.

Storage & Handling

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-human CD4 Monoclonal Antibody Unconjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00344)

Host

Mouse

Contents

PBS pH 7.2, 0.1% (w/v) BSA, 0.09% (w/v) sodium azide

Clonality

Monoclonal

Clone Number

Clone: OKT4

Isotype

IgG2b,k

Immunogen

Human peripheral blood T lymphocytes

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

FC00344 is reactive to CD4 in Cynomolgus monkey, Human, Rhesus monkey

Applications

FC00344 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

51.111kDa

Background of CD4

CD stands for "cluster of differentiation"; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: [T,gp55]. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

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Lymphocyte gated PBMCs stained with purified anti-human CD4 (clone OKT-4) followed by Goat anti-mouse IgG (H+L) Alexa Fluor 488 (red histogram). Lymphocyte gated PBMCs stained with only Goat anti-mouse IgG (H+L) Alexa Fluor 488 (Blue histogram).

Gene/Protein Information For CD4 (Source: Uniprot.org, NCBI)

Alternative Names

CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 CD4 CD4mut CD4 molecule T-cell surface glycoprotein CD4|CD4 antigen (p55)|CD4 receptor|T-cell surface antigen T4/Leu-3

For more info on CD4, check out the CD4 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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