Anti-human CD59 Monoclonal Antibody Biotin Conjugated, Flow Validated

CD59 antibody

Boster Bio Anti-human CD59 Monoclonal Antibody Biotin Conjugated, Flow Validated (Catalog# FC00914-Biotin). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Product Info Summary

SKU: FC00914-Biotin
Size: 100ug
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry

Product Name

Anti-human CD59 Monoclonal Antibody Biotin Conjugated, Flow Validated

View all CD59 Antibodies

SKU/Catalog Number

FC00914-Biotin

Size

100ug

Form

Liquid

Description

Boster Bio Anti-human CD59 Monoclonal Antibody Biotin Conjugated, Flow Validated (Catalog# FC00914-Biotin). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-human CD59 Monoclonal Antibody Biotin Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00914-Biotin)

Host

Mouse

Contents

PBS pH 7.2, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

Clone: BRA-10G

Isotype

IgG1,k

Immunogen

Human K562 tumor cells

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

FC00914-Biotin is reactive to CD59 in Human

Reconstitution

Calculated molecular weight

14177 MW

Background of CD59

This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. And this protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. It also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

FC00914-Biotin is guaranteed for Flow Cytometry Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

Gene/Protein Information For CD59 (Source: Uniprot.org, NCBI)

Gene Name

CD59

Full Name

CD59 glycoprotein

Weight

14177 MW

Alternative Names

CD59 glycoprotein;1F5 antigen;20 kDa homologous restriction factor;HRF-20;HRF20;MAC-inhibitory protein;MAC-IP;MEM43 antigen;Membrane attack complex inhibition factor;MACIF;Membrane inhibitor of reactive lysis;MIRL;Protectin;CD59;CD59;MIC11, MIN1, MIN2, MIN3, MSK21; CD59 16.3A5, 1F5, EJ16, EJ30, EL32, G344, HRF-20, HRF20, MAC-IP, MACIF, MEM43, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20 CD59 molecule (CD59 blood group) CD59 glycoprotein|1F5 |20 kDa homologous restriction factor|CD59 p18-20 ( by antibodies 16.3A5, EJ16, EJ30, EL32 and G344)|CD59 blood group |CD59 molecule, complement regulatory protein|Ly-6-like protein|MEM43 |T cell-activating protein|human leukocyte MIC11|lymphocytic CD59/MEM43|membrane attack complex (MAC) inhibition factor|membrane attack complex inhibition factor|membrane inhibitor of reactive lysis|protectin|surface anitgen recognized by 16.3A5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD59, check out the CD59 Infographic

CD59 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD59: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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10 Customer Q&As for Anti-human CD59 Monoclonal Antibody Biotin Conjugated, Flow Validated

Question

I have a question about product FC00914-Biotin, anti-human CD59 Monoclonal antibody Biotin Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-03-05

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC00914-Biotin anti-human CD59 Monoclonal antibody Biotin Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-05

Question

See below the WB image, lot number and protocol we used for milk using anti-human CD59 Monoclonal antibody Biotin Conjugated, Flow Validated FC00914-Biotin. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-01-15

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-15

Question

Is a blocking peptide available for product anti-human CD59 Monoclonal antibody Biotin Conjugated, Flow Validated (FC00914-Biotin)?

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

We do provide the blocking peptide for product anti-human CD59 Monoclonal antibody Biotin Conjugated, Flow Validated (FC00914-Biotin). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-06

Question

I see that the anti-human CD59 Monoclonal antibody Biotin Conjugated, Flow Validated FC00914-Biotin works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

M. Johnson

Verified customer

Asked: 2019-09-09

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-09

Question

I was wanting to use your anti-human CD59 Monoclonal antibody Biotin Conjugated, Flow Validated for Flow Cytometry for human milk on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human milk identification?

Verified Customer

Verified customer

Asked: 2019-07-10

Answer

You can see on the product datasheet, FC00914-Biotin anti-human CD59 Monoclonal antibody Biotin Conjugated, Flow Validated has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human milk in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-10

Question

Will FC00914-Biotin anti-human CD59 Monoclonal antibody Biotin Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-08

Answer

You can see on the product datasheet, FC00914-Biotin anti-human CD59 Monoclonal antibody Biotin Conjugated, Flow Validated as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-08

Question

We need to test anti-human CD59 Monoclonal antibody Biotin Conjugated, Flow Validated FC00914-Biotin on human milk for research purposes, then I may be interested in using anti-human CD59 Monoclonal antibody Biotin Conjugated, Flow Validated FC00914-Biotin for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-01-22

Answer

The products we sell, including anti-human CD59 Monoclonal antibody Biotin Conjugated, Flow Validated FC00914-Biotin, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-01-22

Question

Is there a BSA free version of anti-human CD59 Monoclonal antibody Biotin Conjugated, Flow Validated FC00914-Biotin available?

Verified Customer

Verified customer

Asked: 2019-01-16

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-human CD59 Monoclonal antibody Biotin Conjugated, Flow Validated FC00914-Biotin are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-01-16

Question

Would anti-human CD59 Monoclonal antibody Biotin Conjugated, Flow Validated FC00914-Biotin work for Flow Cytometry with milk?

Verified Customer

Verified customer

Asked: 2018-07-09

Answer

According to the expression profile of milk, CD59 is highly expressed in milk. So, it is likely that anti-human CD59 Monoclonal antibody Biotin Conjugated, Flow Validated FC00914-Biotin will work for Flow Cytometry with milk.

Boster Scientific Support

Answered: 2018-07-09

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for milk using anti-human CD59 Monoclonal antibody Biotin Conjugated, Flow Validated FC00914-Biotin. Let me know if you need anything else.

J. Moore

Verified customer

Asked: 2015-06-18

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-06-18

$299
Backordered.

Lead time for this item is typically 6-8 days

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In stock
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FC00914-Biotin
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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