Product Info Summary
SKU: | FC00914-PE |
---|---|
Size: | 100 tests, 200 tests |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD59 Monoclonal Antibody PE Conjugated, Flow Validated
SKU/Catalog Number
FC00914-PE
Size
100 tests, 200 tests
Form
Liquid
Description
Boster Bio Anti-human CD59 Monoclonal Antibody PE Conjugated, Flow Validated (Catalog# FC00914-PE). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD59 Monoclonal Antibody PE Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00914-PE)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: BRA-10G
Isotype
IgG1,k
Immunogen
Human K562 tumor cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC00914-PE is reactive to CD59 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
14177 MW
Background of CD59
This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. And this protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. It also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC00914-PE is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
Lymphocytes gated human PBMCs stained with mouse anti-human CD59 PE (clone BRA-10G, red histogram). Blue histogram is for the lymphocytes gated PBMCs stained with mouse IgG1 (MOPC31C) PE conjugated isotype control.
Protein Target Info & Infographic
Gene/Protein Information For CD59 (Source: Uniprot.org, NCBI)
Gene Name
CD59
Full Name
CD59 glycoprotein
Weight
14177 MW
Alternative Names
CD59 glycoprotein;1F5 antigen;20 kDa homologous restriction factor;HRF-20;HRF20;MAC-inhibitory protein;MAC-IP;MEM43 antigen;Membrane attack complex inhibition factor;MACIF;Membrane inhibitor of reactive lysis;MIRL;Protectin;CD59;CD59;MIC11, MIN1, MIN2, MIN3, MSK21; CD59 16.3A5, 1F5, EJ16, EJ30, EL32, G344, HRF-20, HRF20, MAC-IP, MACIF, MEM43, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20 CD59 molecule (CD59 blood group) CD59 glycoprotein|1F5 |20 kDa homologous restriction factor|CD59 p18-20 ( by antibodies 16.3A5, EJ16, EJ30, EL32 and G344)|CD59 blood group |CD59 molecule, complement regulatory protein|Ly-6-like protein|MEM43 |T cell-activating protein|human leukocyte MIC11|lymphocytic CD59/MEM43|membrane attack complex (MAC) inhibition factor|membrane attack complex inhibition factor|membrane inhibitor of reactive lysis|protectin|surface anitgen recognized by 16.3A5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD59, check out the CD59 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD59: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD59 Monoclonal Antibody PE Conjugated, Flow Validated (FC00914-PE)
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Customer Q&As
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2 Customer Q&As for Anti-human CD59 Monoclonal Antibody PE Conjugated, Flow Validated
Question
I was wanting to use your anti-human CD59 Monoclonal antibody PE Conjugated, Flow Validated for Flow Cytometry for human colon on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human colon identification?
J. Patel
Verified customer
Asked: 2018-07-27
Answer
You can see on the product datasheet, FC00914-PE anti-human CD59 Monoclonal antibody PE Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human colon in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-27
Question
I am interested in to test anti-human CD59 Monoclonal antibody PE Conjugated, Flow Validated FC00914-PE on human colon for research purposes, then I may be interested in using anti-human CD59 Monoclonal antibody PE Conjugated, Flow Validated FC00914-PE for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Yang
Verified customer
Asked: 2015-06-15
Answer
The products we sell, including anti-human CD59 Monoclonal antibody PE Conjugated, Flow Validated FC00914-PE, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-06-15