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Product Info Summary
| SKU: | RP1023 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Growth Hormone/GH1 Antibody Picoband®
SKU/Catalog Number
RP1023
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Growth Hormone/GH1 Antibody catalog # RP1023. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Growth Hormone/GH1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1023)
Host
Rabbit
Contents
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human GH recombinant protein (Position: F27-F217).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1023 is reactive to GH1 in Human
Observed Molecular Weight
22 kDa
Calculated molecular weight
24.8 kDa
Background of GH1
Growth Hormone (GH) is mapped to 17q22-q24. Growth hormone (GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. It binds two receptor molecules and thereby induces signal transduction through receptor dimerization. At high concentrations, GH acts as an antagonist because of a large difference in affinities at the respective binding sites.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1023 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human placenta tissue
IHC: Human Placenta Tissue
Validation Images & Assay Conditions
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Anti-human Growth Hormone antibody, RP1023, IHC(P)
IHC(P): Human Placenta Tissue
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Western blot analysis of GH1 using anti-GH1 antibody (RP1023).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GH1 antigen affinity purified polyclonal antibody (Catalog # RP1023) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GH1 at approximately 22KD. The expected band size for GH1 is at 25KD.
Specific Publications For Anti-Growth Hormone/GH1 Antibody Picoband® (RP1023)
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