Anti-Human ICOS DyLight® 488 conjugated Antibody

ICOS antibody

Boster Bio Anti-Human ICOS DyLight® 488 conjugated Antibody catalog # A00291-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: A00291-Dyl488
Size: 50ug/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry

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Product Name

Anti-Human ICOS DyLight® 488 conjugated Antibody

View all ICOS Antibodies

SKU/Catalog Number

A00291-Dyl488

Size

50ug/vial

Form

Liquid

Description

Boster Bio Anti-Human ICOS DyLight® 488 conjugated Antibody catalog # A00291-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At 2-8?C for one year from date of receipt. Protect from light. Do not freeze.

Cite This Product

Anti-Human ICOS DyLight® 488 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00291-Dyl488)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human ICOS recombinant protein (Position: E21-K140).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00291-Dyl488 is reactive to ICOS in Human

Applications

A00291-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee

Background of ICOS

Inducible T-cell costimulator is an immune checkpoint protein that in humans is encoded by the ICOS gene. The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For ICOS (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9Y6W8

Gene ID

29851

Gene Name

ICOS

Full Name

Inducible T-cell costimulator

Weight

22.625kDa

Alternative Names

Activation-inducible lymphocyte immunomediatory molecule; AILIM; AILIMCD278; CD278 antigen; CD278; CRP-1; CVID1; ICOS; inducible costimulator; inducible T-cell costimulator; inducible T-cell co-stimulator; MGC39850 ICOS AILIM, CD278, CVID1 inducible T cell costimulator inducible T-cell costimulator|activation-inducible lymphocyte immunomediatory molecule|inducible T-cell co-stimulator|inducible costimulator

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ICOS, check out the ICOS Infographic

ICOS infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ICOS: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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14 Customer Q&As for Anti-Human ICOS DyLight® 488 conjugated Antibody

Question

We need to test anti-Human ICOS DyLight® 488 conjugated antibody A00291-Dyl488 on human blood for research purposes, then I may be interested in using anti-Human ICOS DyLight® 488 conjugated antibody A00291-Dyl488 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

The products we sell, including anti-Human ICOS DyLight® 488 conjugated antibody A00291-Dyl488, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-04

Question

I have attached the WB image, lot number and protocol we used for blood using anti-Human ICOS DyLight® 488 conjugated antibody A00291-Dyl488. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-28

Question

Our lab were happy with the WB result of your anti-Human ICOS DyLight® 488 conjugated antibody. However we have observed positive staining in leukocyte isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is ICOS supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-21

Answer

From literature, leukocyte does express ICOS. Generally ICOS expresses in isoform 1: cell membrane. Regarding which tissues have ICOS expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 11006126, 15489334
Thymus, Pubmed ID: 10779774

Boster Scientific Support

Answered: 2019-10-21

Question

Is a blocking peptide available for product anti-Human ICOS DyLight® 488 conjugated antibody (A00291-Dyl488)?

Verified Customer

Verified customer

Asked: 2019-10-04

Answer

We do provide the blocking peptide for product anti-Human ICOS DyLight® 488 conjugated antibody (A00291-Dyl488). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-10-04

Question

We have seen staining in human leukocyte. Are there any suggestions? Is anti-Human ICOS DyLight® 488 conjugated antibody supposed to stain leukocyte positively?

M. Williams

Verified customer

Asked: 2019-09-26

Answer

According to literature leukocyte does express ICOS. According to Uniprot.org, ICOS is expressed in leukocyte, blood, thymus, among other tissues. Regarding which tissues have ICOS expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 11006126, 15489334
Thymus, Pubmed ID: 10779774

Boster Scientific Support

Answered: 2019-09-26

Question

Is this A00291-Dyl488 anti-Human ICOS DyLight® 488 conjugated antibody reactive to the isotypes of ICOS?

Verified Customer

Verified customer

Asked: 2019-09-25

Answer

The immunogen of A00291-Dyl488 anti-Human ICOS DyLight® 488 conjugated antibody is E. coli-derived human ICOS recombinant protein (Position: E21-K140). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-25

Question

My question regarding product A00291-Dyl488, anti-Human ICOS DyLight® 488 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-11

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00291-Dyl488 anti-Human ICOS DyLight® 488 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-11

Question

We are currently using anti-Human ICOS DyLight® 488 conjugated antibody A00291-Dyl488 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?

A. Li

Verified customer

Asked: 2019-03-01

Answer

The anti-Human ICOS DyLight® 488 conjugated antibody (A00291-Dyl488) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-03-01

Question

Would A00291-Dyl488 anti-Human ICOS DyLight® 488 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-02-13

Answer

It shows on the product datasheet, A00291-Dyl488 anti-Human ICOS DyLight® 488 conjugated antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-02-13

Question

Will anti-Human ICOS DyLight® 488 conjugated antibody A00291-Dyl488 work for Flow Cytometry with blood?

Verified Customer

Verified customer

Asked: 2019-02-05

Answer

According to the expression profile of blood, ICOS is highly expressed in blood. So, it is likely that anti-Human ICOS DyLight® 488 conjugated antibody A00291-Dyl488 will work for Flow Cytometry with blood.

Boster Scientific Support

Answered: 2019-02-05

Question

I was wanting to use your anti-Human ICOS DyLight® 488 conjugated antibody for Flow Cytometry for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?

Verified Customer

Verified customer

Asked: 2018-12-04

Answer

As indicated on the product datasheet, A00291-Dyl488 anti-Human ICOS DyLight® 488 conjugated antibody has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-12-04

Question

I see that the anti-Human ICOS DyLight® 488 conjugated antibody A00291-Dyl488 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-09-04

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-09-04

Question

Do you have a BSA free version of anti-Human ICOS DyLight® 488 conjugated antibody A00291-Dyl488 available?

Verified Customer

Verified customer

Asked: 2017-07-07

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Human ICOS DyLight® 488 conjugated antibody A00291-Dyl488 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-07-07

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-Human ICOS DyLight® 488 conjugated antibody A00291-Dyl488. Let me know if you need anything else.

O. Zhang

Verified customer

Asked: 2016-05-27

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-05-27

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A00291-Dyl488
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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