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Product Info Summary
| SKU: | M08374 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Human IgG3 IGHG3 Monoclonal Antibody
SKU/Catalog Number
M08374
BM5333 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Human IgG3 IGHG3 Monoclonal Antibody catalog # M08374. Tested in WB, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IgG3 IGHG3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08374)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACIA-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Human IgG3 IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%) .
Reactive Species
M08374 is reactive to IGHG3 in Human
Observed Molecular Weight
55 kDa
Calculated molecular weight
41.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08374 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Positive Control
WB: human plasma
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Human IgG3 expression in human plasma lysate.
Specific Publications For Anti-Human IgG3 IGHG3 Monoclonal Antibody (M08374)
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Customer Q&As
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7 Customer Q&As for Anti-Human IgG3 IGHG3 Monoclonal Antibody
Question
Is there a BSA free version of anti-Human IgG3 Monoclonal antibody M08374 available?
S. Anderson
Verified customer
Asked: 2020-01-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human IgG3 Monoclonal antibody M08374 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-30
Question
Here is the WB image, lot number and protocol we used for liver using anti-Human IgG3 Monoclonal antibody M08374. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-02
Question
I was wanting to use your anti-Human IgG3 Monoclonal antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
It shows on the product datasheet, M08374 anti-Human IgG3 Monoclonal antibody has been tested for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-23
Question
I would like to test anti-Human IgG3 Monoclonal antibody M08374 on human liver for research purposes, then I may be interested in using anti-Human IgG3 Monoclonal antibody M08374 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
The products we sell, including anti-Human IgG3 Monoclonal antibody M08374, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-15
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Human IgG3 Monoclonal antibody M08374. Let me know if you need anything else.
M. Wu
Verified customer
Asked: 2018-01-22
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-22
Question
Is this M08374 anti-Human IgG3 Monoclonal antibody reactive to the isotypes of IGHG3?
Verified Customer
Verified customer
Asked: 2017-07-27
Answer
The immunogen of M08374 anti-Human IgG3 Monoclonal antibody is A synthesized peptide derived from human Human IgG3 IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%) . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-27
Question
Does anti-Human IgG3 Monoclonal antibody M08374 work for WB with liver?
B. Carter
Verified customer
Asked: 2015-04-30
Answer
According to the expression profile of liver, IGHG3 is highly expressed in liver. So, it is likely that anti-Human IgG3 Monoclonal antibody M08374 will work for WB with liver.
Boster Scientific Support
Answered: 2015-04-30
