Anti-Human NFATC4 DyLight® 550 conjugated Antibody

NFATC4 antibody

Boster Bio Anti-Human NFATC4 DyLight® 550 conjugated Antibody catalog # A02599-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: A02599-Dyl550
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry

Product Name

Anti-Human NFATC4 DyLight® 550 conjugated Antibody

View all NFATC4 Antibodies

SKU/Catalog Number

A02599-Dyl550

Size

100 μg/vial

Form

Liquid

Description

Boster Bio Anti-Human NFATC4 DyLight® 550 conjugated Antibody catalog # A02599-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human NFATC4 DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02599-Dyl550)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human NFATC4 recombinant protein (Position: Q413-D522).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02599-Dyl550 is reactive to NFATC4 in Human

Applications

A02599-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

95.449kDa

Background of NFATC4

Nuclear factor of activated T-cells, cytoplasmic 4 is a protein that in humans is encoded by the NFATC4 gene. This gene encodes a member of the nuclear factor of activated T cells (NFAT) protein family. The encoded protein is part of a DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor stimulation and an inducible nuclear component. NFAT proteins are activated by the calmodulin-dependent phosphatase, calcineurin. The encoded protein plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of interleukin-2 and interleukin-4.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For NFATC4 (Source: Uniprot.org, NCBI)

Gene Name

NFATC4

Full Name

Nuclear factor of activated T-cells, cytoplasmic 4

Weight

95.449kDa

Alternative Names

NF-AT3; NFAT3nuclear factor of activated T-cells, cytoplasmic 4; NFATc4; NF-ATc4; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4; T cell transcription factor NFAT3; T-cell transcription factor NFAT3 NFATC4 NF-AT3, NF-ATC4, NFAT3 nuclear factor of activated T cells 4 nuclear factor of activated T-cells, cytoplasmic 4|T-cell transcription factor NFAT3|nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NFATC4, check out the NFATC4 Infographic

NFATC4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NFATC4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A02599-Dyl550

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Human NFATC4 DyLight® 550 conjugated Antibody?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

Be the first to review Anti-Human NFATC4 DyLight® 550 conjugated Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

5 Customer Q&As for Anti-Human NFATC4 DyLight® 550 conjugated Antibody

Question

We are currently using anti-Human NFATC4 DyLight® 550 conjugated antibody A02599-Dyl550 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-18

Answer

The anti-Human NFATC4 DyLight® 550 conjugated antibody (A02599-Dyl550) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-18

Question

Is this A02599-Dyl550 anti-Human NFATC4 DyLight® 550 conjugated antibody reactive to the isotypes of NFATC4?

A. Zhang

Verified customer

Asked: 2019-08-05

Answer

The immunogen of A02599-Dyl550 anti-Human NFATC4 DyLight® 550 conjugated antibody is E. coli-derived human NFATC4 recombinant protein (Position: Q413-D522). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-05

Question

Can you help my question with product A02599-Dyl550, anti-Human NFATC4 DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-30

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02599-Dyl550 anti-Human NFATC4 DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-30

Question

Does anti-Human NFATC4 DyLight® 550 conjugated antibody A02599-Dyl550 work on zebrafish Flow Cytometry with ovary rhabdomyosarcoma?

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

Our lab technicians have not tested anti-Human NFATC4 DyLight® 550 conjugated antibody A02599-Dyl550 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Human NFATC4 DyLight® 550 conjugated antibody A02599-Dyl550 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish ovary rhabdomyosarcoma in Flow Cytometry, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-24

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Human NFATC4 DyLight® 550 conjugated antibody A02599-Dyl550. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-07-20

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-20

Order DetailsPrice
A02599-Dyl550

100μg

$515
A02599-Dyl550-carrier-free

Carrier Free

$515

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
A02599-Dyl550
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$515.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.