Anti-Human RAB27A DyLight® 550 conjugated Antibody

RAB27A antibody

Boster Bio Anti-Human RAB27A DyLight® 550 conjugated Antibody catalog # A01608-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: A01608-Dyl550
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry

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Product Name

Anti-Human RAB27A DyLight® 550 conjugated Antibody

View all RAB27A Antibodies

SKU/Catalog Number

A01608-Dyl550

Size

100 μg/vial

Form

Liquid

Description

Boster Bio Anti-Human RAB27A DyLight® 550 conjugated Antibody catalog # A01608-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human RAB27A DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01608-Dyl550)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human RAB27A recombinant protein (Position: L98-K216).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01608-Dyl550 is reactive to RAB27A in Human

Applications

A01608-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee

Background of RAB27A

Ras-related protein Rab-27A is a protein that in humans is encoded by the RAB27A gene. The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For RAB27A (Source: Uniprot.org, NCBI)

Uniprot ID

P51159

Gene ID

5873

Gene Name

RAB27A

Full Name

Ras-related protein Rab-27A

Weight

24.868kDa

Superfamily

small GTPase superfamily

Alternative Names

GS2; GS2rab-27; GTP-binding protein Ram; HsT18676; Rab-27; Rab27a; RAB27A, member RAS oncogene family; RAB27MGC117246; RAM; RAMras-related protein Rab-27A RAB27A GS2, HsT18676, RAB27, RAM RAB27A, member RAS oncogene family ras-related protein Rab-27A|GTP-binding protein Ram|mutant Ras-related protein Rab-27A|rab-27

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RAB27A, check out the RAB27A Infographic

RAB27A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RAB27A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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6 Customer Q&As for Anti-Human RAB27A DyLight® 550 conjugated Antibody

Question

Is this A01608-Dyl550 anti-Human RAB27A DyLight® 550 conjugated antibody reactive to the isotypes of RAB27A?

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

The immunogen of A01608-Dyl550 anti-Human RAB27A DyLight® 550 conjugated antibody is E. coli-derived human RAB27A recombinant protein (Position: L98-K216). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-05-01

Question

Does A01608-Dyl550 anti-Human RAB27A DyLight® 550 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-01-24

Answer

As indicated on the product datasheet, A01608-Dyl550 anti-Human RAB27A DyLight® 550 conjugated antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-01-24

Question

Please see the WB image, lot number and protocol we used for mammary gland using anti-Human RAB27A DyLight® 550 conjugated antibody A01608-Dyl550. Please let me know if you require anything else.

G. Carter

Verified customer

Asked: 2018-01-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-24

Question

Our lab want to know about to test anti-Human RAB27A DyLight® 550 conjugated antibody A01608-Dyl550 on human mammary gland for research purposes, then I may be interested in using anti-Human RAB27A DyLight® 550 conjugated antibody A01608-Dyl550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-07-31

Answer

The products we sell, including anti-Human RAB27A DyLight® 550 conjugated antibody A01608-Dyl550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-07-31

Question

Is a blocking peptide available for product anti-Human RAB27A DyLight® 550 conjugated antibody (A01608-Dyl550)?

N. Krishna

Verified customer

Asked: 2015-07-14

Answer

We do provide the blocking peptide for product anti-Human RAB27A DyLight® 550 conjugated antibody (A01608-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-07-14

Question

I see that the anti-Human RAB27A DyLight® 550 conjugated antibody A01608-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

N. Banerjee

Verified customer

Asked: 2013-04-03

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-04-03

Size

Total: $515

SKU:A01608-Dyl550

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Ships directly to USA and Canada. For other regions, visit our distributors page

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A01608-Dyl550
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$515.00

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