Product Info Summary
SKU: | A01608-Dyl550 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human RAB27A DyLight® 550 conjugated Antibody
SKU/Catalog Number
A01608-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human RAB27A DyLight® 550 conjugated Antibody catalog # A01608-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human RAB27A DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01608-Dyl550)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human RAB27A recombinant protein (Position: L98-K216).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01608-Dyl550 is reactive to RAB27A in Human
Applications
A01608-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
24.868kDa
Background of RAB27A
Ras-related protein Rab-27A is a protein that in humans is encoded by the RAB27A gene. The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry, 1-3μg/1x106 cells
Validation Images & Assay Conditions
Click image to see more details
Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For RAB27A (Source: Uniprot.org, NCBI)
Gene Name
RAB27A
Full Name
Ras-related protein Rab-27A
Weight
24.868kDa
Superfamily
small GTPase superfamily
Alternative Names
GS2; GS2rab-27; GTP-binding protein Ram; HsT18676; Rab-27; Rab27a; RAB27A, member RAS oncogene family; RAB27MGC117246; RAM; RAMras-related protein Rab-27A RAB27A GS2, HsT18676, RAB27, RAM RAB27A, member RAS oncogene family ras-related protein Rab-27A|GTP-binding protein Ram|mutant Ras-related protein Rab-27A|rab-27
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB27A, check out the RAB27A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB27A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human RAB27A DyLight® 550 conjugated Antibody (A01608-Dyl550)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Human RAB27A DyLight® 550 conjugated Antibody
Question
Is this A01608-Dyl550 anti-Human RAB27A DyLight® 550 conjugated antibody reactive to the isotypes of RAB27A?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
The immunogen of A01608-Dyl550 anti-Human RAB27A DyLight® 550 conjugated antibody is E. coli-derived human RAB27A recombinant protein (Position: L98-K216). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-05-01
Question
Does A01608-Dyl550 anti-Human RAB27A DyLight® 550 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-01-24
Answer
As indicated on the product datasheet, A01608-Dyl550 anti-Human RAB27A DyLight® 550 conjugated antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-24
Question
Please see the WB image, lot number and protocol we used for mammary gland using anti-Human RAB27A DyLight® 550 conjugated antibody A01608-Dyl550. Please let me know if you require anything else.
G. Carter
Verified customer
Asked: 2018-01-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-24
Question
Our lab want to know about to test anti-Human RAB27A DyLight® 550 conjugated antibody A01608-Dyl550 on human mammary gland for research purposes, then I may be interested in using anti-Human RAB27A DyLight® 550 conjugated antibody A01608-Dyl550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-07-31
Answer
The products we sell, including anti-Human RAB27A DyLight® 550 conjugated antibody A01608-Dyl550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-31
Question
Is a blocking peptide available for product anti-Human RAB27A DyLight® 550 conjugated antibody (A01608-Dyl550)?
N. Krishna
Verified customer
Asked: 2015-07-14
Answer
We do provide the blocking peptide for product anti-Human RAB27A DyLight® 550 conjugated antibody (A01608-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-07-14
Question
I see that the anti-Human RAB27A DyLight® 550 conjugated antibody A01608-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
N. Banerjee
Verified customer
Asked: 2013-04-03
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-04-03