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Product Info Summary
| SKU: | A01281-Dyl550 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human TAPA1 DyLight® 550 conjugated CD81 Antibody
SKU/Catalog Number
A01281-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human TAPA1 DyLight® 550 conjugated CD81 Antibody catalog # A01281-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human TAPA1 DyLight® 550 conjugated CD81 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01281-Dyl550)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TAPA1 recombinant protein (Position: F113-K201).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01281-Dyl550 is reactive to CD81 in Human
Applications
A01281-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
25.809kDa
Background of CD81
CD81 molecule, also known as CD81 (Cluster of Differentiation 81), is a protein which in humans is encoded by the CD81 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry, 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For CD81 (Source: Uniprot.org, NCBI)
Gene Name
CD81
Full Name
CD81 antigen
Weight
25.809kDa
Superfamily
tetraspanin (TM4SF) family
Alternative Names
CD81 antigen (target of antiproliferative 1); CD81 antigen; CD81 molecule; CD81; CVID6; TAPA1; TAPA-1; Target of the antiproliferative 1; Tetraspanin-28; TSPAN28; Tspan-28; tspan-28,26 kDa cell surface protein TAPA-1; TSPAN28S5.7 CD81 CVID6, S5.7, TAPA1, TSPAN28 CD81 molecule CD81 antigen|26 kDa cell surface protein TAPA-1|CD81 antigen (target of antiproliferative antibody 1)|tetraspanin-28|tspan-28
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD81, check out the CD81 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD81: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human TAPA1 DyLight® 550 conjugated CD81 Antibody (A01281-Dyl550)
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No publications found for A01281-Dyl550
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Human TAPA1 DyLight® 550 conjugated CD81 Antibody
Question
I was wanting to use your anti-Human TAPA1 DyLight® 550 conjugated antibody for Flow Cytometry for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
You can see on the product datasheet, A01281-Dyl550 anti-Human TAPA1 DyLight® 550 conjugated antibody has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-27
Question
We want to test anti-Human TAPA1 DyLight® 550 conjugated antibody A01281-Dyl550 on human brain for research purposes, then I may be interested in using anti-Human TAPA1 DyLight® 550 conjugated antibody A01281-Dyl550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
The products we sell, including anti-Human TAPA1 DyLight® 550 conjugated antibody A01281-Dyl550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-05
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Human TAPA1 DyLight® 550 conjugated antibody A01281-Dyl550. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-06
Question
Do you have a BSA free version of anti-Human TAPA1 DyLight® 550 conjugated antibody A01281-Dyl550 available?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Human TAPA1 DyLight® 550 conjugated antibody A01281-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-03
Question
Is a blocking peptide available for product anti-Human TAPA1 DyLight® 550 conjugated antibody (A01281-Dyl550)?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
We do provide the blocking peptide for product anti-Human TAPA1 DyLight® 550 conjugated antibody (A01281-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-17
Question
Does anti-Human TAPA1 DyLight® 550 conjugated antibody A01281-Dyl550 work for Flow Cytometry with brain?
N. Lewis
Verified customer
Asked: 2019-08-26
Answer
According to the expression profile of brain, CD81 is highly expressed in brain. So, it is likely that anti-Human TAPA1 DyLight® 550 conjugated antibody A01281-Dyl550 will work for Flow Cytometry with brain.
Boster Scientific Support
Answered: 2019-08-26
Question
We are currently using anti-Human TAPA1 DyLight® 550 conjugated antibody A01281-Dyl550 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
The anti-Human TAPA1 DyLight® 550 conjugated antibody (A01281-Dyl550) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-29
Question
Is this A01281-Dyl550 anti-Human TAPA1 DyLight® 550 conjugated antibody reactive to the isotypes of CD81?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
The immunogen of A01281-Dyl550 anti-Human TAPA1 DyLight® 550 conjugated antibody is E. coli-derived human TAPA1 recombinant protein (Position: F113-K201). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-03
Question
My question regarding product A01281-Dyl550, anti-Human TAPA1 DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Baker
Verified customer
Asked: 2019-05-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01281-Dyl550 anti-Human TAPA1 DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-24
Question
I see that the anti-Human TAPA1 DyLight® 550 conjugated antibody A01281-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-28
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-03-28
Question
We have observed staining in human myometrium. Are there any suggestions? Is anti-Human TAPA1 DyLight® 550 conjugated antibody supposed to stain myometrium positively?
Verified Customer
Verified customer
Asked: 2019-02-14
Answer
From literature myometrium does express CD81. From Uniprot.org, CD81 is expressed in myometrium, brain, among other tissues. Regarding which tissues have CD81 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-02-14
Question
We were well pleased with the WB result of your anti-Human TAPA1 DyLight® 550 conjugated antibody. However we have seen positive staining in myometrium cell membrane using this antibody. Is that expected? Could you tell me where is CD81 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-12-11
Answer
From what I have seen in literature, myometrium does express CD81. Generally CD81 expresses in cell membrane. Regarding which tissues have CD81 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-12-11
Question
I have attached the WB image, lot number and protocol we used for brain using anti-Human TAPA1 DyLight® 550 conjugated antibody A01281-Dyl550. Please let me know if you require anything else.
S. Carter
Verified customer
Asked: 2017-12-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-01
Question
Does A01281-Dyl550 anti-Human TAPA1 DyLight® 550 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
As indicated on the product datasheet, A01281-Dyl550 anti-Human TAPA1 DyLight® 550 conjugated antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-11
