Anti-Huntingtin/HTT Antibody

Product Name

Anti-Huntingtin/HTT Antibody

View all Huntingtin Antibodies

SKU/Catalog Number

PA2268

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Huntingtin/HTT Antibody catalog # PA2268. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Huntingtin/HTT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2268)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Huntingtin, different from the related mouse and rat sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2268 is reactive to HTT in Human, Mouse, Rat

Applications

PA2268 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

348 kDa

Calculated molecular weight

347.603kDa

Background of Huntingtin

The huntingtin gene, also called HTT or HD (Huntington disease) gene, is the IT15 ("interesting transcript 15") gene which codes for a protein called the huntingtin protein. It is mapped to 4p16.3. The protein has no sequence homology with other proteins and is highly expressed in neurons and tests in humans and rodents. HTT upregulates the expression of Brain Derived Neurotrophic Factor (BDNF) at the transcription level, and this gene is required for normal development. The HTT protein is involved in vesicle trafficking as it interacts with HIP1, a clathrin-binding protein, to mediate endocytosis, the absorption of materials into a cell. HTT was also visualized as punctate staining likely to represent nerve endings. What's more, wildtype HTT may function in the nucleus in the assembly of nuclear matrix-bound protein complexes involved with transcriptional repression and RNA processing.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Anti-Huntingtin antibody, PA2268, All Western blotting
All lanes: Anti-HTT(PA2268) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Predicted bind size: 348KD
Observed bind size: 348KD

Gene/Protein Information For HTT (Source: Uniprot.org, NCBI)

Alternative Names

HD protein; huntingtin (Huntington disease); huntingtin; IT15HDHuntington disease protein HTT HD, IT15, LOMARS huntingtin huntingtin|huntington disease protein

For more info on HTT, check out the HTT Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HTT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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