Product Info Summary
SKU: | A00900 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IF, WB |
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Product info
Product Name
Anti-hUPF1 Antibody
View all RENT1/UPF1/hUPF1 Antibodies
SKU/Catalog Number
A00900
Size
100ul
Form
Liquid
Description
Boster Bio Anti-hUPF1 Antibody catalog # A00900. Tested in WB,IF applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-hUPF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00900)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CALR.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00900 is reactive to UPF1 in Human, Mouse
Applications
A00900 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
124.345kDa
Background of RENT1/UPF1/hUPF1
Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis By similarity.
McCauliffe D.P., J. Clin. Invest. 85:1379-1391(1990).
Rokeach L.A., J. Immunol. 147:3031-3039(1991).
McCauliffe D.P., J. Biol. Chem. 267:2557-2562(1992).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of UPF1 using Anti-hUPF1 Antibody (A00900).
ICC staining hUPF1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-hUPF1 Antibody A00900
Western blot analysis of hUPF1 on PC-3M cell lysate using anti-hUPF1 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For UPF1 (Source: Uniprot.org, NCBI)
Gene Name
UPF1
Full Name
Regulator of nonsense transcripts 1
Weight
124.345kDa
Superfamily
DNA2/NAM7 helicase family
Alternative Names
ATP-dependent helicase RENT1; EC 3.6.1; EC 3.6.4.-; FLJ43809; HUPF1; KIAA0221FLJ46894; Nonsense mRNA reducing factor 1; NORF1; NORF1delta helicase; pNORF1; regulator of nonsense transcripts 1; RENT1; RENT1UP Frameshift 1; UPF1 regulator of nonsense transcripts homolog (yeast); UPF1; up-frameshift mutation 1 homolog; Up-frameshift suppressor 1 homolog; yeast Upf1p homolog UPF1 HUPF1, NORF1, RENT1, pNORF1, smg-2 UPF1 RNA helicase and ATPase regulator of nonsense transcripts 1|ATP-dependent helicase RENT1|UPF1 regulator of nonsense transcripts homolog|delta helicase|nonsense mRNA reducing factor 1|smg-2 homolog, nonsense mediated mRNA decay factor|up-frameshift mutation 1 homolog|up-frameshift suppressor 1 homolog|yeast Upf1p homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UPF1, check out the UPF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UPF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-hUPF1 Antibody (A00900)
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No publications found for A00900
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Customer Reviews
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