Anti-HuR / ELAVL1 Monoclonal Antibody

Product Name

Anti-HuR / ELAVL1 Monoclonal Antibody

View all HuR/ELAVL1 Antibodies

SKU/Catalog Number

M00736

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-HuR / ELAVL1 Monoclonal Antibody catalog # M00736. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HuR / ELAVL1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00736)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ADHI-5

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human HuR / ELAVL1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00736 is reactive to ELAVL1 in Human, Mouse, Rat

Applications

M00736 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

36 kDa

Calculated molecular weight

36.092kDa

Background of HuR/ELAVL1

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:40
FC 1:50

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ELAVL1 using anti-ELAVL1 antibody (M00736).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human MOLT-4 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELAVL1 antigen affinity purified monoclonal antibody (Catalog # M00736) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ELAVL1 at approximately 36 kDa. The expected band size for ELAVL1 is at 36 kDa.

Gene/Protein Information For ELAVL1 (Source: Uniprot.org, NCBI)

Alternative Names

ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R); ELAV-like protein 1; embryonic lethal, abnormal vision, drosophila, homolog-like 1; Hu antigen R; Hua; Hu-antigen R; HuR; HURELAV1; MelG ELAVL1 ELAV1, HUR, Hua, MelG ELAV like RNA binding protein 1 ELAV-like protein 1|ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)|Hu antigen R|embryonic lethal, abnormal vision, drosophila, homolog-like 1|hu-antigen R

For more info on ELAVL1, check out the ELAVL1 Infographic

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In this infographic, you will see the following information for ELAVL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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