Click image to see more details
Product Info Summary
| SKU: | M01562 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IAP2 BIRC3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01562
BM4056 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IAP2 BIRC3 Rabbit Monoclonal Antibody catalog # M01562. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IAP2 BIRC3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01562)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BOB-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IAP2
Reactive Species
M01562 is reactive to BIRC3 in Human
Observed Molecular Weight
72 kDa
Calculated molecular weight
68.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01562 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: Ramos cell
Validation Images & Assay Conditions
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Western blot analysis of IAP2 expression in Ramos cell lysate.
Specific Publications For Anti-IAP2 BIRC3 Rabbit Monoclonal Antibody (M01562)
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Customer Q&As
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4 Customer Q&As for Anti-IAP2 BIRC3 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-IAP2 Rabbit Monoclonal antibody (M01562)?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
We do provide the blocking peptide for product anti-IAP2 Rabbit Monoclonal antibody (M01562). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-22
Question
See attached the WB image, lot number and protocol we used for fetal liver using anti-IAP2 Rabbit Monoclonal antibody M01562. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-25
Question
Would M01562 anti-IAP2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
It shows on the product datasheet, M01562 anti-IAP2 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-28
Question
I have a question about product M01562, anti-IAP2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Krishna
Verified customer
Asked: 2013-11-22
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01562 anti-IAP2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-11-22
