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Product Info Summary
| SKU: | A11221 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-IF3 MTIF3 Antibody
SKU/Catalog Number
A11221
Size
100μl
Form
Liquid
Description
Boster Bio Anti-IF3 MTIF3 Antibody catalog # A11221. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IF3 MTIF3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11221)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MTIF3. AA range:69-118
Reactive Species
A11221 is reactive to MTIF3 in Human, Mouse
Calculated molecular weight
31.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11221 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of HuvEC cells using IF3 Polyclonal Antibody diluted at 1:500
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-IF3 MTIF3 Antibody (A11221)
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Customer Q&As
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7 Customer Q&As for Anti-IF3 MTIF3 Antibody
Question
Does anti-IF3 antibody A11221 work for WB with cervix colon?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
According to the expression profile of cervix colon, MTIF3 is highly expressed in cervix colon. So, it is likely that anti-IF3 antibody A11221 will work for WB with cervix colon.
Boster Scientific Support
Answered: 2020-03-17
Question
I have a question about product A11221, anti-IF3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11221 anti-IF3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-30
Question
We are currently using anti-IF3 antibody A11221 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
The anti-IF3 antibody (A11221) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-27
Question
Is a blocking peptide available for product anti-IF3 antibody (A11221)?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
We do provide the blocking peptide for product anti-IF3 antibody (A11221). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-16
Question
Is this A11221 anti-IF3 antibody reactive to the isotypes of MTIF3?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
The immunogen of A11221 anti-IF3 antibody is Synthesized peptide derived from the Internal region of human IF3. at AA rangle: 40-120. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-20
Question
Will anti-IF3 antibody A11221 work on primate WB with melanocyte?
D. Krishna
Verified customer
Asked: 2015-04-08
Answer
Our lab technicians have not validated anti-IF3 antibody A11221 on primate. You can run a BLAST between primate and the immunogen sequence of anti-IF3 antibody A11221 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate melanocyte in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-04-08
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix colon using anti-IF3 antibody A11221. Let me know if you need anything else.
J. Carter
Verified customer
Asked: 2013-09-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-09-23
