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Product Info Summary
| SKU: | A02041-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-IFN-beta IFNB1 Antibody
SKU/Catalog Number
A02041-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IFN-beta IFNB1 Antibody (Catalog # A02041-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
IFN-beta antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-IFN-beta IFNB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02041-1)
Host
Rabbit
Contents
IFN-beta Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-IFN-beta antibody was raised against a peptide corresponding to 17 amino acids near the center of human IFN-beta. The immunogen is located within amino acids 110-160 of IFN-beta.
Reactive Species
A02041-1 is reactive to IFNB1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
22.3 kDa
Background of IFNB1
Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by "Natural IFN-producing cells" (IPCs) as part of the host immune response and can also inhibit the development of tumors. IFN-beta binding by its receptor results in the activation of the tyrosine kinases Jak1 and Tyk2 and phosphorylation of members of the STAT family of transcription factors, leading to the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02041-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 4-10 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation in Mouse A20 Cell Lysate
Loading: 15 μg of lysates per lane.
Antibodies: IFN-beta A02041-1 (Lane 1: 5 μg/mL and Lane 2: 10 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: IFN-beta A02041-1 (4 μg/mL), IFN-beta 64-239, (5 μg/mL) and beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Multiple Tissues and Recombinant Protein
Loading: 15 μg of lysates per lane.
Antibodies: IFN-beta A02041-1 (4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunohistochemistry Validation of IFN-beta in Human Liver Tissue
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IFN-beta antibody (A02041-1) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of IFN-beta in Human Liver Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed human liver cells labeling IFN-beta with A02041-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Immunohistochemistry Validation of IFN-beta in Human Liver Tissue
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IFN-beta antibody (A02041-1) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of IFN-beta in Mouse Liver Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling IFN-beta with A02041-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Specific Publications For Anti-IFN-beta IFNB1 Antibody (A02041-1)
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