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Product Info Summary
| SKU: | M00393-5 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Mouse |
| Host: | Rat |
| Application: | IHC-P |
Product info
Product Name
Anti- IFN Gamma Monoclonal Antibody
SKU/Catalog Number
M00393-5
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti- IFN Gamma Monoclonal Antibody catalog # M00393-5. Tested in IHC-P applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti- IFN Gamma Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00393-5)
Host
Rat
Contents
PBS, pH 7.0. Contains no stabilizers or preservatives
Clonality
Monoclonal
Clone Number
AFII-9
Isotype
Rat IgG1, κ
Immunogen
Recombinant mouse IFNγ
Cross-reactivity
No cross reactivity with other proteins
Reactive Species
M00393-5 is reactive to Ifng in Mouse
Calculated molecular weight
17.9 kDa
Background of Ifng
Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00393-5 is guaranteed for IHC-P Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse
Positive Control
IHC: mouse lymphaden tissue
Validation Images & Assay Conditions
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IHC analysis of IFN Gamma using anti-IFN Gamma antibody (M00393-5).
IFN Gamma was detected in paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml rat anti-IFN Gamma Antibody (M00393-5) overnight at 4°C. Biotinylated goat anti-rat IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Specific Publications For Anti- IFN Gamma Monoclonal Antibody (M00393-5)
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