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- Table of Contents
Ifng antibody (M00393-5) performs well in IHC-P and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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IHC analysis of IFN Gamma using anti-IFN Gamma antibody (M00393-5).
IFN Gamma was detected in paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml rat anti-IFN Gamma Antibody (M00393-5) overnight at 4°C. Biotinylated goat anti-rat IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
IFN Gamma was detected in paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml rat anti-IFN Gamma Antibody (M00393-5) overnight at 4°C. Biotinylated goat anti-rat IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Designing a Western blot for IFNG? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
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Anti- IFN Gamma Monoclonal Antibody
Ifng antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rat | IHC-P |
Tested Applications
Ifng antibody was positively detected by IHC in:
mouse lymphaden tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Product Information
Product Name
Anti- IFN Gamma Monoclonal Antibody
View all IFNG Antibodies
View all IFNG Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rat
Contents
PBS, pH 7.0. Contains no stabilizers or preservatives
Clonality
Monoclonal
Clone Number
AFII-9
Isotype
Rat IgG1, κ
Immunogen
Recombinant mouse IFNγ
Cross-Reactivity
No cross reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Calculated Molecular Weight
17.9 kDa
Background of Ifng
Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Cite This Product
Anti- IFN Gamma Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00393-5)
Protocols
Immunohistochemistry (IHC) protocol for Ifng antibody
Publications
Application:
Species:
Combination of mesenchymal stem cells and FK506 prolongs heart allograft survival by inhibiting TBK1/IRF3-regulated-IFN-γ production
Published: 2021-07-04
Journal: IMMUNOLOGY LETTERS
Application: IF
Impact Factor: 3.69
Species: Mouse
PMID: 34228988
Human interleukin-10 gene inhibits acute rejection by triggering apoptosis in allograft vascular transplantation
Published: 2014-08-15
Journal: International Journal of Clinical and Experimental Pathology
Application: IHC
Impact Factor: 1.78
Species: Rat
PMID: 25337228
Effect of Iodine Excess on Th1, Th2, Th17, and Treg Cell Subpopulations in the Thyroid of NOD.H-2h4 Mice
Published: 2014-04-17
Journal: BIOLOGICAL TRACE ELEMENT RESEARCH
Application: IHC
Impact Factor: 1.61
Species: Mouse
PMID: 24740393
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Product Promise
We promise Anti- IFN Gamma Monoclonal Antibody performs excellently in relevant applications for IHC-P. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00393-5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 20, 2026