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Product Info Summary
| SKU: | A00393 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-IFN-Gamma (Q69) IFNG Antibody
SKU/Catalog Number
A00393
Size
100ul
Form
Liquid
Description
Boster Bio Anti-IFN-Gamma (Q69) IFNG Antibody catalog # A00393. Tested in WB,IHC,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IFN-Gamma (Q69) IFNG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00393)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 31-80 of Human IFN-γ.
Cross-reactivity
Does not cross-react with eNOS or nNOS.
Reactive Species
A00393 is reactive to IFNG in Human, Mouse, Rat
Calculated molecular weight
19.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00393 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IP: 1:50-1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of IFN-γ (Q69) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate
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Immunohistochemistry (IHC) analyzes of IFN-γ (Q69) pAb in paraffin-embedded human lymph node tissue.
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Immunoprecipitation of the Spleen tissue lysate of Rat using IFN-γ (Q69) pAb (Sepharose Bead Conjugate) #BD0047(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using IFN-γ (Q69) pAb #BS3486.“↑”(supernatant); “↓”(deposition)
Specific Publications For Anti-IFN-Gamma (Q69) IFNG Antibody (A00393)
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