Anti-Ikaros/IKZF1 Antibody Picoband™ (monoclonal, 5F12H7)

Product Name

Anti-Ikaros/IKZF1 Antibody Picoband™ (monoclonal, 5F12H7)

View all Ikaros/IKZF1 Antibodies

SKU/Catalog Number

M00531-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Ikaros/IKZF1 Antibody Picoband™ (monoclonal, 5F12H7) catalog # M00531-3. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Ikaros/IKZF1 Antibody Picoband™ (monoclonal, 5F12H7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00531-3)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

5F12H7

Isotype

IgG1

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Ikaros, different from the related mouse sequence by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M00531-3 is reactive to IKZF1 in Human

Applications

M00531-3 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

55-65 kDa

Calculated molecular weight

57.528kDa

Background of Ikaros/IKZF1

DNA-binding protein Ikaros is a protein that in humans is encoded by the IKZF1 gene. This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Ikaros/IKZF1 using anti-Ikaros/IKZF1 antibody (M00531-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Ikaros/IKZF1 antigen affinity purified monoclonal antibody (Catalog # M00531-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Ikaros/IKZF1 at approximately 55-65 kDa. The expected band size for Ikaros/IKZF1 is at 58 kDa.

Gene/Protein Information For IKZF1 (Source: Uniprot.org, NCBI)

Alternative Names

DNA-binding protein Ikaros; hIk-1; IK1; IK1LyF-1; Ikaros (zinc finger protein); IKAROS family zinc finger 1 (Ikaros); Ikaros family zinc finger protein 1; Ikaros; IKAROSLymphoid transcription factor LyF-1; IKZF1; LYF1; LyF-1; LYF1PRO0758; PRO0758; zinc finger protein, subfamily 1A, 1 (Ikaros); ZNFN1A1; ZNFN1A1CLL-associated antigen KW-6 IKZF1 CVID13, Hs.54452, IK1, IKAROS, LYF1, LyF-1, PPP1R92, PRO0758, ZNFN1A1 IKAROS family zinc finger 1 DNA-binding protein Ikaros|CLL-associated antigen KW-6|ikaros family zinc finger protein 1|lymphoid transcription factor LyF-1|protein phosphatase 1, regulatory subunit 92|zinc finger protein, subfamily 1A, 1 (Ikaros)

For more info on IKZF1, check out the IKZF1 Infographic

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In this infographic, you will see the following information for IKZF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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