Anti-IKB beta/NFKBIB Antibody Picoband™

Product Name

Anti-IKB beta/NFKBIB Antibody Picoband™

View all IkB-beta Antibodies

SKU/Catalog Number

PB9292

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IKB beta/NFKBIB Antibody Picoband™ catalog # PB9292. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IKB beta/NFKBIB Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9292)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human IKB beta recombinant protein (Position: E56-E237). Human IKB beta shares 82% and 80% amino acid (aa) sequence identity with mouse and rat IKB beta, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9292 is reactive to NFKBIB in Human, Mouse, Rat

Applications

PB9292 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

38 kDa

Calculated molecular weight

37.771kDa

Background of IkB-beta

NF-kappa-B inhibitor beta, also known as IKBB or TRIP9, is a protein that in humans is encoded by the NFKBIB gene. The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. This gene is mapped to 19q13.2. It has been found that in vivo, NFKBIB serves both to inhibit and to facilitate the inflammatory response. NFKBIB degradation releases NF-kappa-B dimers, which upregulate proinflammatory target genes such as TNF-alpha. Surprisingly, absence of NFKBIB results in a dramatic reduction of TNF-alpha in response to lipopolysaccharide, even though activation of NF-kappa-B is normal.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Anti-IKB beta Picoband antibody, PB9292, Western blotting
All lanes: Anti IKB beta (PB9292) at 0.5ug/ml
Lane 1: Mouse Kidney Tissue Lysate at 50ug
Lane 2: RH35 Whole Cell Lysate at 40ug
Lane 3: NRK Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD

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Anti-IKB beta Picoband antibody, PB9292,IHC(P)
IHC(P): Human Intestinal Cancer Tissue

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Anti-IKB beta Picoband antibody, PB9292,IHC(P)
IHC(P): Mouse Intestine Tissue

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Anti-IKB beta Picoband antibody, PB9292,IHC(P)
IHC(P): Rat Intestine Tissue

Gene/Protein Information For NFKBIB (Source: Uniprot.org, NCBI)

Alternative Names

beta; IkappaBbeta; I-kappa-B-beta; ikB-B; IkBbeta; IkB-beta; IKBBikB-beta; NF-kappa-B inhibitor beta; NF-kappa-BIB; NFKBIB; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP-9; TRIP9ikappaBbeta NFKBIB IKBB, TRIP9 NFKB inhibitor beta NF-kappa-B inhibitor beta|I-kappa-B-beta|NF-kappa-BIB|TR-interacting protein 9|TRIP-9|ikB-B|ikB-beta|ikappaBbeta|nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta|thyroid receptor-interacting protein 9

For more info on NFKBIB, check out the NFKBIB Infographic

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In this infographic, you will see the following information for NFKBIB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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