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- Table of Contents
Facts about NF-kappa-B inhibitor beta.
Association with inhibitor kappa B- interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation making it more resistant to degradation, describing its slower degradation. .
Human | |
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Gene Name: | NFKBIB |
Uniprot: | Q15653 |
Entrez: | 4793 |
Belongs to: |
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NF-kappa-B inhibitor family |
beta; IkappaBbeta; I-kappa-B-beta; ikB-B; IkBbeta; IkB-beta; IKBBikB-beta; NF-kappa-B inhibitor beta; NF-kappa-BIB; NFKBIB; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP-9; TRIP9ikappaBbeta
Mass (kDA):
37.771 kDA
Human | |
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Location: | 19q13.2 |
Sequence: | 19; NC_000019.10 (38899503..38908894) |
Expressed in all tissues examined.
Cytoplasm. Nucleus.
If you're looking for a high affinity primary antibody to recognize NFKBIB, you've probably come across Steven Boster's work. While it is impossible to fully understand Boster's work, he has been a renowned researcher for 25 years, and his antibodies have been extensively cited by the research community. They are also validated for use in ELISA, Western Blotting, and Immunohistochemistry.
In the field of biotechnology, Boster Bio offers a comprehensive portfolio of high-affinity primary antibodies. These antibodies are validated on Western Blotting, Immunohistochemistry, and ELISA platforms, which have earned the company widespread reputation. Boster products are available in a wide range of applications, including neuroscience and cancer research. With a focus on quality and consistency, Boster antibodies are the choice of researchers around the world.
The Boster Bio high-affinity primary antibodies were created by a team of scientists from several universities. Mariana Lemos Duarte designed and performed the experiments, developed the CRISPR/Cas9 knock-out cells, validated the antibodies, and wrote the manuscript. Nikita Trimbake and Achla Gupta collaborated on the project, and Xiaomin Fan developed yeast antibody display technology. The team also developed peptide sequences for protein antigens.
In addition to their excellent affinity to ricin, all Boster Bio high-affinity primary antibodies showed excellent neutralization potency in vitro. They also showed excellent protection against ricin in post-exposure studies. In vitro, these antibodies are highly effective in neutralizing ricin, despite having a low affinity for the toxin. Further research will try to determine these antibodies' dissociation constants using biophysical methods.
In addition, the H5-Ab2 and H5-Ab4 were highly specific for gp120. The I45R/T49R mutants impaired binding to H5-Ab2 and H5-Ab4 antibodies. Hence, the Boster Bio high-affinity primary antibodies are essential in immunohistochemistry. Therefore, you should invest in these antibodies for the benefit of your research.
To test the affinity of these antibody clones, the protein of interest should be expressed in the target cell. A full-length rabbit antibody is produced by ExpiCHO cells and purified by protein A column chromatography. The sample was then buffer exchanged into PBS. Afterward, the concentration of the IgG was measured by the Lowry's assay. In the case of cPKC-specific antibodies, a phosphospecific antibody is synthesized from the C2-domain of the target protein.
Another characteristic of the c-chain of these antigens is the unique sequences of k and l light chains. These clones are isolated from diverse germline genes. Five IGHV genes and seven IGHD genes were isolated from the same library. Three IGLJ genes were isolated from the same library as the five IGHV gene. The k light chain, on the other hand, is cloned from three IGLV genes and one IGLJ gene.
Several mAbs are prepared by immunostaining, which is prone to bias. High-throughput microscopy eliminates these concerns while being time and cost-effective. This method involves seeding cells of interest on 96-well plates and image acquisition using an IN Cell microscope (GE Healthcare).
The NFKBIB gene is part of the NF family of transcription factors, which play a role in immunity. The NFKBIB marker is used to determine the activity of these genes, including a number of genes that are involved in immunity. In the Boster bio, we have found a canonical NFKBIB signature, which is consistent with USRs in TLR signaling. The dominant canonical NFKBIA signature likely reflects the activities of the K1L and A49R genes, which prevent NFKBIA from degradation and bind ubiquitin ligase.
PMID: 7776974 by Lee J.W., et al. Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor.
PMID: 8816457 by Suyang H., et al. Role of unphosphorylated, newly synthesized IkappaB beta in persistent activation of NF-kappaB.