Product Info Summary
SKU: | A07073 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-IkB-e(Ab-22) NFKBIE Antibody
View all IkB-epsilon Antibodies
SKU/Catalog Number
A07073
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-IkB-e(Ab-22) NFKBIE Antibody (Catalog # A07073). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IkB-e(Ab-22) NFKBIE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07073)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa. 20~24 (I-E-S-L-R) derived from Human IkB-e.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07073 is reactive to NFKBIE in Human, Mouse, Rat
Applications
A07073 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
52.864kDa
Background of IkB-epsilon
Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
Shirane M, et al. (1999) J Biol Chem; 274(40): 28169-74
Karin, M. and Ben-Neriah, Y. (2000) Annu. Rev. Immunol. ;18, 621-663.
Chen, Z.J. et al. (1996) Cell; 84, 853-862.
Brown, K. et al. (1995) Science; 267, 1485-1488.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 40kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from Hela, 293 and HepG2 cells using IkB-e(Ab-22) antibody A07073.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-e(Ab-22) antibody A07073(left) or the same antibody preincubated with blocking peptide(right).
Protein Target Info & Infographic
Gene/Protein Information For NFKBIE (Source: Uniprot.org, NCBI)
Gene Name
NFKBIE
Full Name
NF-kappa-B inhibitor epsilon
Weight
52.864kDa
Superfamily
NF-kappa-B inhibitor family
Alternative Names
epsilon; ikappaBepsilon; I-kappa-B-epsilon; IkB-E; IKBENF-kappa-B inhibitor epsilon; IkBepsilon; IkB-epsilon; NF-kappa-BIE; NFKBIE; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor NFKBIE IKBE NFKB inhibitor epsilon NF-kappa-B inhibitor epsilon|I-kappa-B-epsilon|NF-kappa-BIE|ikB-E|ikB-epsilon|ikappaBepsilon|nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFKBIE, check out the NFKBIE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFKBIE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IkB-e(Ab-22) NFKBIE Antibody (A07073)
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No publications found for A07073
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Customer Reviews
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