Anti-IKB epsilon NFKBIE Monoclonal Antibody

IkB-epsilon antibody

Boster Bio Anti-IKB epsilon NFKBIE Monoclonal Antibody catalog # M07073. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.

Product Info Summary

SKU: M07073
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

Product Name

Anti-IKB epsilon NFKBIE Monoclonal Antibody

View all IkB-epsilon Antibodies

SKU/Catalog Number

M07073

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-IKB epsilon NFKBIE Monoclonal Antibody catalog # M07073. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IKB epsilon NFKBIE Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07073)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ADCF-14

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human IKB epsilon Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M07073 is reactive to NFKBIE in Human

Applications

M07073 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

116 kDa

Calculated molecular weight

52.864kDa

Background of IkB-epsilon

Putative transcription factor involved in pancreas development and function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For NFKBIE (Source: Uniprot.org, NCBI)

Gene Name

NFKBIE

Full Name

NF-kappa-B inhibitor epsilon

Weight

52.864kDa

Superfamily

NF-kappa-B inhibitor family

Alternative Names

epsilon; ikappaBepsilon; I-kappa-B-epsilon; IkB-E; IKBENF-kappa-B inhibitor epsilon; IkBepsilon; IkB-epsilon; NF-kappa-BIE; NFKBIE; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor NFKBIE IKBE NFKB inhibitor epsilon NF-kappa-B inhibitor epsilon|I-kappa-B-epsilon|NF-kappa-BIE|ikB-E|ikB-epsilon|ikappaBepsilon|nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NFKBIE, check out the NFKBIE Infographic

NFKBIE infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NFKBIE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-IKB epsilon NFKBIE Monoclonal Antibody

Question

Will anti-IKB epsilon Monoclonal antibody M07073 work on bovine IP with testis?

Verified Customer

Verified customer

Asked: 2020-01-14

Answer

Our lab technicians have not validated anti-IKB epsilon Monoclonal antibody M07073 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-IKB epsilon Monoclonal antibody M07073 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine testis in IP, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-14

Question

Would anti-IKB epsilon Monoclonal antibody M07073 work for IP with b-cell?

Verified Customer

Verified customer

Asked: 2019-10-28

Answer

According to the expression profile of b-cell, NFKBIE is highly expressed in b-cell. So, it is likely that anti-IKB epsilon Monoclonal antibody M07073 will work for IP with b-cell.

Boster Scientific Support

Answered: 2019-10-28

Question

I am interested in to test anti-IKB epsilon Monoclonal antibody M07073 on human b-cell for research purposes, then I may be interested in using anti-IKB epsilon Monoclonal antibody M07073 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-03-12

Answer

The products we sell, including anti-IKB epsilon Monoclonal antibody M07073, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-03-12

Question

I have a question about product M07073, anti-IKB epsilon Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

L. Bhatt

Verified customer

Asked: 2018-11-15

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M07073 anti-IKB epsilon Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-11-15

Question

Is this M07073 anti-IKB epsilon Monoclonal antibody reactive to the isotypes of NFKBIE?

H. Bhatt

Verified customer

Asked: 2017-05-02

Answer

The immunogen of M07073 anti-IKB epsilon Monoclonal antibody is A synthesized peptide derived from human IKB epsilon Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-05-02

Question

We are currently using anti-IKB epsilon Monoclonal antibody M07073 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?

E. Wu

Verified customer

Asked: 2014-04-22

Answer

The anti-IKB epsilon Monoclonal antibody (M07073) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-04-22

Order DetailsPrice
M07073

100μL

$370
M07073-30ul

30μL sample

$99
M07073-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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M07073
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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