Anti-IKB epsilon/Nfkbie Antibody Picoband™

Product Name

Anti-IKB epsilon/Nfkbie Antibody Picoband™

View all IkB-epsilon Antibodies

SKU/Catalog Number

A07073-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IKB epsilon/Nfkbie Antibody Picoband™ catalog # A07073-1. Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-IKB epsilon/Nfkbie Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07073-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse IKB epsilon/Nfkbie recombinant protein (Position: Q111-D364).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07073-1 is reactive to NFKBIE in Mouse

Applications

A07073-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

41 kDa

Calculated molecular weight

52.864kDa

Background of IkB-epsilon

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon, also known as NFKBIE, is a protein which in humans is encoded by the NFKBIE gene. Predicted to be involved in D-serine transport. Predicted to act upstream of or within negative regulation of transcription, DNA-templated. Predicted to be located in several cellular components, including Golgi apparatus; nuclear lumen; and perinuclear region of cytoplasm. Is expressed in several structures, including central nervous system; dorsal root ganglion; eye; genitourinary system; and thymus. Orthologous to human NFKBIE (NFKB inhibitor epsilon).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml/ml, Mouse
Direct ELISA, 0.1-0.5 μg/ml/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of IKB epsilon/Nfkbie using anti-IKB epsilon/Nfkbie antibody (A07073-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKB epsilon/Nfkbie antigen affinity purified polyclonal antibody (Catalog # A07073-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKB epsilon/Nfkbie at approximately 41 kDa. The expected band size for IKB epsilon/Nfkbie is at 53 kDa.

Gene/Protein Information For NFKBIE (Source: Uniprot.org, NCBI)

Alternative Names

epsilon; ikappaBepsilon; I-kappa-B-epsilon; IkB-E; IKBENF-kappa-B inhibitor epsilon; IkBepsilon; IkB-epsilon; NF-kappa-BIE; NFKBIE; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor NFKBIE IKBE NFKB inhibitor epsilon NF-kappa-B inhibitor epsilon|I-kappa-B-epsilon|NF-kappa-BIE|ikB-E|ikB-epsilon|ikappaBepsilon|nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon

For more info on NFKBIE, check out the NFKBIE Infographic

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In this infographic, you will see the following information for NFKBIE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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