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Product Info Summary
| SKU: | A00118 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-IKK beta IKBKB Antibody
SKU/Catalog Number
A00118
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-IKK beta IKBKB Antibody (Catalog # A00118). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-IKK beta IKBKB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00118)
Host
Rabbit
Contents
0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
IKKb peptide corresponding to the highly conserved C-terminus region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Reactive Species
A00118 is reactive to IKBKB in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
86.6 kDa
Background of IKBKB
NFkB comprises a family of cellular transcription factors that are involved in the inducible expression of a variety of cellular genes that regulate the inflammatory response and control of cell death. In the cytoplasm NFkB is negatively modulated by the inhibitory proteins IkB. In turn IkB is phosphorylated by a cellular kinase complex called IKK. IKK is a heterodimer composed of two kinases: IKK-a and IKK-b that phosphorylate IkB leading to its degradation and the resulting translocation of NFkB to the nucleus. IKK kinase activity is modulated negatively by pharmaceutical agents such as aspirin and positively by various cellular components such as TNF- a, endotoxins and overexpression of cellular kinases like MEKK1. Aspirin appears to have its effect by inhibiting the binding of ATP to IKK.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00118 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:1,000 - 1:5,000
IHC: 1:200 - 1:1,000
WB: 1:200 - 1:1,000
This product was tested by immunoblot and found to be reactive against IKK-b at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302. Anti-IKKb is suitable for the detection by immunoblot of human, mouse and rat IKKb showing an 87 kDa band. Anti-IKKb has been tested in IHC using human placenta tissue.
Validation Images & Assay Conditions
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Immunohistochemistry of Anti-IKKß antibody. Tissue: human placenta was formalin fixed and paraffin embedded. No pre-treatment of sample was required. Primary Antibody: Anti-IKKß was diluted 1:500 to detect IKKß in tissue. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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Western blot of anti-IKKß antibody reacted with HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:500 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1h. Washes with TBS preceded color development.
Specific Publications For Anti-IKK beta IKBKB Antibody (A00118)
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