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- Table of Contents
Il17a antibody (M00421-2) performs well in IHC-P and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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IHC analysis of IL17A using anti-IL17A antibody (M00421-2).
IL17A was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml mouse anti-IL17A Antibody (M00421-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
IL17A was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml mouse anti-IL17A Antibody (M00421-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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Anti- IL 17A Monoclonal Antibody
Il17a antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Mouse | IHC-P |
Tested Applications
Il17a antibody was positively detected by IHC in:
mouse spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Product Information
Product Name
Anti- IL 17A Monoclonal Antibody
View all IL17A Antibodies
View all IL17A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
PBS, pH 7.0. Contains no stabilizers or preservatives
Clonality
Monoclonal
Clone Number
AFIF-9
Isotype
Mouse IgG1, κ
Immunogen
Mouse IL-17A cross-linked to OVA
Cross-Reactivity
No cross reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
42 kDa
Calculated Molecular Weight
17.5 kDa
Background of Il17a
Interleukin-17A is a protein that in humans is encoded by the IL17A gene. The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Cite This Product
Anti- IL 17A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00421-2)
Protocols
Immunohistochemistry (IHC) protocol for Il17a antibody
Publications
Application:
Species:
CCL17-neutralizing and esterase-responsive core-shell microgels for endogenous Tregs recruitment and functional enhancement in myocardial infarction
Published: 2026-06-16
Journal: Bioactive Materials
Application: IF
Impact Factor: 23.60
Species: Mouse
PMID: 42359364
Pirfenidone ameliorates silica-induced lung inflammation and fibrosis in mice by inhibiting the secretion of interleukin-17A
Published: 2021-07-27
Journal: ACTA PHARMACOLOGICA SINICA
Impact Factor: 6.15
Species: Mouse
PMID: 34316030
CD4+, IL17 and Foxp3 Expression in Different pTNM Stages of Operable Non-small Cell Lung Cancer and Effects on Disease Prognosis
Published: 2012-08-31
Journal: Asian Pacific Journal of Cancer Prevention
Application: IHC
Impact Factor: 0.66
Species: Human
PMID: 23098499
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Product Promise
We promise Anti- IL 17A Monoclonal Antibody performs excellently in relevant applications for IHC-P. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00421-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026